Sensors 2013, 13(7), 8490-8500; doi:10.3390/s130708490
Article

Transverse Susceptibility as a Biosensor for Detection of Au-Fe3O4 Nanoparticle-Embedded Human Embryonic Kidney Cells

1 Department of Physics, University of South Florida, Tampa, FL 33620, USA 2 Department of Internal Medicine, University of South Florida, Tampa, FL 33620, USA 3 Department of Medical Laboratory Sciences, University of Delaware, Newark, DE 19716, USA
* Authors to whom correspondence should be addressed.
Received: 19 April 2013; in revised form: 24 June 2013 / Accepted: 25 June 2013 / Published: 3 July 2013
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Abstract: We demonstrate the possibility of using a radio-frequency transverse susceptibility (TS) technique based on a sensitive self-resonant tunnel-diode oscillator as a biosensor for detection of cancer cells that have taken up magnetic nanoparticles. This technique can detect changes in frequency on the order of 10 Hz in 10 MHz. Therefore, a small sample of cells that have taken up nanoparticles when placed inside the sample space of the TS probe can yield a signal characteristic of the magnetic nanoparticles. As a proof of the concept, Fe3O4 nanoparticles coated with Au (mean size ~60 nm) were synthesized using a micellar method and these nanoparticles were introduced to the medium at different concentrations of 0.05, 0.1, 0.5, and 1 mg/mL buffer, where they were taken up by human embryonic kidney (HEK) cells via phagocytosis. While the highest concentration of Au-Fe3O4 nanoparticles (1 mg/mL) was found to give the strongest TS signal, it is notable that the TS signal of the nanoparticles could still be detected at concentrations as low as 0.1 mg/mL.
Keywords: magnetic nanoparticles; cells; magnetic biosensors

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MDPI and ACS Style

Huls, N.F.; Phan, M.-H.; Kumar, A.; Mohapatra, S.; Mohapatra, S.; Mukherjee, P.; Srikanth, H. Transverse Susceptibility as a Biosensor for Detection of Au-Fe3O4 Nanoparticle-Embedded Human Embryonic Kidney Cells. Sensors 2013, 13, 8490-8500.

AMA Style

Huls NF, Phan M-H, Kumar A, Mohapatra S, Mohapatra S, Mukherjee P, Srikanth H. Transverse Susceptibility as a Biosensor for Detection of Au-Fe3O4 Nanoparticle-Embedded Human Embryonic Kidney Cells. Sensors. 2013; 13(7):8490-8500.

Chicago/Turabian Style

Huls, Natalie F.; Phan, Manh-Huong; Kumar, Arun; Mohapatra, Subhra; Mohapatra, Shyam; Mukherjee, Pritish; Srikanth, Hariharan. 2013. "Transverse Susceptibility as a Biosensor for Detection of Au-Fe3O4 Nanoparticle-Embedded Human Embryonic Kidney Cells." Sensors 13, no. 7: 8490-8500.

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