Next Article in Journal
A Robust Approach for a Filter-Based Monocular Simultaneous Localization and Mapping (SLAM) System
Next Article in Special Issue
Rapid Quantitative Detection of Brucella melitensis by a Label-Free Impedance Immunosensor Based on a Gold Nanoparticle-Modified Screen-Printed Carbon Electrode
Previous Article in Journal
Simulation of a Geiger-Mode Imaging LADAR System for Performance Assessment
Previous Article in Special Issue
Enhanced Electronic Properties of Pt@Ag Heterostructured Nanoparticles
Sensors 2013, 13(7), 8490-8500; doi:10.3390/s130708490
Article

Transverse Susceptibility as a Biosensor for Detection of Au-Fe3O4 Nanoparticle-Embedded Human Embryonic Kidney Cells

1
,
1,* , 2,3
,
2
,
2
,
1
 and
1,*
1 Department of Physics, University of South Florida, Tampa, FL 33620, USA 2 Department of Internal Medicine, University of South Florida, Tampa, FL 33620, USA 3 Department of Medical Laboratory Sciences, University of Delaware, Newark, DE 19716, USA
* Authors to whom correspondence should be addressed.
Received: 19 April 2013 / Revised: 24 June 2013 / Accepted: 25 June 2013 / Published: 3 July 2013
View Full-Text   |   Download PDF [652 KB, uploaded 21 June 2014]   |   Browse Figures
SciFeed

Abstract

We demonstrate the possibility of using a radio-frequency transverse susceptibility (TS) technique based on a sensitive self-resonant tunnel-diode oscillator as a biosensor for detection of cancer cells that have taken up magnetic nanoparticles. This technique can detect changes in frequency on the order of 10 Hz in 10 MHz. Therefore, a small sample of cells that have taken up nanoparticles when placed inside the sample space of the TS probe can yield a signal characteristic of the magnetic nanoparticles. As a proof of the concept, Fe3O4 nanoparticles coated with Au (mean size ~60 nm) were synthesized using a micellar method and these nanoparticles were introduced to the medium at different concentrations of 0.05, 0.1, 0.5, and 1 mg/mL buffer, where they were taken up by human embryonic kidney (HEK) cells via phagocytosis. While the highest concentration of Au-Fe3O4 nanoparticles (1 mg/mL) was found to give the strongest TS signal, it is notable that the TS signal of the nanoparticles could still be detected at concentrations as low as 0.1 mg/mL.
Keywords: magnetic nanoparticles; cells; magnetic biosensors magnetic nanoparticles; cells; magnetic biosensors
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
RIS
MDPI and ACS Style

Huls, N.F.; Phan, M.-H.; Kumar, A.; Mohapatra, S.; Mohapatra, S.; Mukherjee, P.; Srikanth, H. Transverse Susceptibility as a Biosensor for Detection of Au-Fe3O4 Nanoparticle-Embedded Human Embryonic Kidney Cells. Sensors 2013, 13, 8490-8500.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert