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Metaphase FISH on a Chip: Miniaturized Microfluidic Device for Fluorescence in situ Hybridization
DTU-Nanotech, Department of Micro and Nanotechnology, Technical University of Denmark, Kgs. Lyngby 2800, Denmark
Wilhelm Johannsen Centre for Functional Genome Research, Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen, Denmark
Kennedy Center, Gl. Landevej, 2600 Glostrup, Denmark
Both authors contributed equally to this paper and therefore order of authorship is arbitrary.
* Authors to whom correspondence should be addressed.
Received: 2 August 2010; in revised form: 23 September 2010 / Accepted: 15 October 2010 / Published: 2 November 2010
Abstract: Fluorescence in situ Hybridization (FISH) is a major cytogenetic technique for clinical genetic diagnosis of both inherited and acquired chromosomal abnormalities. Although FISH techniques have evolved and are often used together with other cytogenetic methods like CGH, PRINS and PNA-FISH, the process continues to be a manual, labour intensive, expensive and time consuming technique, often taking over 3–5 days, even in dedicated labs. We have developed a novel microFISH device to perform metaphase FISH on a chip which overcomes many shortcomings of the current laboratory protocols. This work also introduces a novel splashing device for preparing metaphase spreads on a microscope glass slide, followed by a rapid adhesive tape-based bonding protocol leading to rapid fabrication of the microFISH device. The microFISH device allows for an optimized metaphase FISH protocol on a chip with over a 20-fold reduction in the reagent volume. This is the first demonstration of metaphase FISH on a microfluidic device and offers a possibility of automation and significant cost reduction of many routine diagnostic tests of genetic anomalies.
Keywords: Fluorescence in situ Hybridization (FISH); lab on chip; genetic analysis; cytogenetics; chromosome spreading; metaphase FISH; chromosomal translocations
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MDPI and ACS Style
Vedarethinam, I.; Shah, P.; Dimaki, M.; Tumer, Z.; Tommerup, N.; Svendsen, W.E. Metaphase FISH on a Chip: Miniaturized Microfluidic Device for Fluorescence in situ Hybridization. Sensors 2010, 10, 9831-9846.
Vedarethinam I, Shah P, Dimaki M, Tumer Z, Tommerup N, Svendsen WE. Metaphase FISH on a Chip: Miniaturized Microfluidic Device for Fluorescence in situ Hybridization. Sensors. 2010; 10(11):9831-9846.
Vedarethinam, Indumathi; Shah, Pranjul; Dimaki, Maria; Tumer, Zeynep; Tommerup, Niels; Svendsen, Winnie E. 2010. "Metaphase FISH on a Chip: Miniaturized Microfluidic Device for Fluorescence in situ Hybridization." Sensors 10, no. 11: 9831-9846.