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Int. J. Mol. Sci. 2017, 18(5), 1037; doi:10.3390/ijms18051037

Selective Expression of Flt3 within the Mouse Hematopoietic Stem Cell Compartment

1
Institute of Immunology and Immunotherapy, College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
2
Developmental and Molecular Immunology, Department of Biomedicine, University of Basel, Basel 4058, Switzerland
3
Institute of Clinical Sciences, College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
*
Author to whom correspondence should be addressed.
Academic Editor: Johannes Haybaeck
Received: 20 April 2017 / Revised: 5 May 2017 / Accepted: 5 May 2017 / Published: 12 May 2017
(This article belongs to the Special Issue The Biology and Treatment of Myeloid Leukaemias)
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Abstract

The fms-like tyrosine kinase 3 (Flt3) is a cell surface receptor that is expressed by various hematopoietic progenitor cells (HPC) and Flt3-activating mutations are commonly present in acute myeloid and lymphoid leukemias. These findings underscore the importance of Flt3 to steady-state and malignant hematopoiesis. In this study, the expression of Flt3 protein and Flt3 mRNA by single cells within the hematopoietic stem cell (HSC) and HPC bone marrow compartments of C57/BL6 mice was investigated using flow cytometry and the quantitative reverse transcription polymerase chain reaction. Flt3 was heterogeneously expressed by almost all of the populations studied, including long-term reconstituting HSC and short-term reconstituting HSC. The erythropoietin receptor (EpoR) and macrophage colony-stimulating factor receptor (M-CSFR) were also found to be heterogeneously expressed within the multipotent cell compartments. Co-expression of the mRNAs encoding Flt3 and EpoR rarely occurred within these compartments. Expression of both Flt3 and M-CSFR protein at the surface of single cells was more commonly observed. These results emphasize the heterogeneous nature of HSC and HPC and the new sub-populations identified are important to understanding the origin and heterogeneity of the acute myeloid leukemias. View Full-Text
Keywords: Flt3; growth factor receptors; cytokines; HSC; hematopoiesis; leukemia Flt3; growth factor receptors; cytokines; HSC; hematopoiesis; leukemia
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MDPI and ACS Style

Mooney, C.J.; Cunningham, A.; Tsapogas, P.; Toellner, K.-M.; Brown, G. Selective Expression of Flt3 within the Mouse Hematopoietic Stem Cell Compartment. Int. J. Mol. Sci. 2017, 18, 1037.

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