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Int. J. Mol. Sci. 2014, 15(12), 22492-22517; doi:10.3390/ijms151222492

Comprehensive Analysis of Neonatal versus Adult Unilateral Decortication in a Mouse Model Using Behavioral, Neuroanatomical, and DNA Microarray Approaches

1
Department of Anatomy, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, Japan
2
Department of Physical Therapy, Faculty of Health Science, Tokoha University, 1-30 Mizuochicho, Aoi, Shizuoka 420-0831, Japan
3
Laboratory of Regulatory Biology, Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan
4
Laboratory of Exercise Biochemistry and Neuroendocrinology, Institute of Health and Sports Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba 305-8574, Japan
5
Organization for Educational Initiatives, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba 305-8577, Japan
*
Authors to whom correspondence should be addressed.
Received: 3 October 2014 / Revised: 17 November 2014 / Accepted: 20 November 2014 / Published: 5 December 2014
(This article belongs to the Special Issue Neurological Injuries’ Monitoring, Tracking and Treatment)
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Abstract

Previously, studying the development, especially of corticospinal neurons, it was concluded that the main compensatory mechanism after unilateral brain injury in rat at the neonatal stage was due in part to non-lesioned ipsilateral corticospinal neurons that escaped selection by axonal elimination or neuronal apoptosis. However, previous results suggesting compensatory mechanism in neonate brain were not correlated with high functional recovery. Therefore, what is the difference among neonate and adult in the context of functional recovery and potential mechanism(s) therein? Here, we utilized a brain unilateral decortication mouse model and compared motor functional recovery mechanism post-neonatal brain hemisuction (NBH) with adult brain hemisuction (ABH). Three analyses were performed: (1) Quantitative behavioral analysis of forelimb movements using ladder walking test; (2) neuroanatomical retrograde tracing analysis of unlesioned side corticospinal neurons; and (3) differential global gene expressions profiling in unlesioned-side neocortex (rostral from bregma) in NBH and ABH on a 8 × 60 K mouse whole genome Agilent DNA chip. Behavioral data confirmed higher recovery ability in NBH over ABH is related to non-lesional frontal neocortex including rostral caudal forelimb area. A first inventory of differentially expressed genes genome-wide in the NBH and ABH mouse model is provided as a resource for the scientific community. View Full-Text
Keywords: neonatal; adult; brain; motor functional recovery; DNA microarray; 8 × 60 K; transcriptome profiling; gene inventory neonatal; adult; brain; motor functional recovery; DNA microarray; 8 × 60 K; transcriptome profiling; gene inventory
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Yoshikawa, A.; Nakamachi, T.; Shibato, J.; Rakwal, R.; Shioda, S. Comprehensive Analysis of Neonatal versus Adult Unilateral Decortication in a Mouse Model Using Behavioral, Neuroanatomical, and DNA Microarray Approaches. Int. J. Mol. Sci. 2014, 15, 22492-22517.

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