Next Article in Journal
Spectroscopic Studies of Model Photo-Receptors: Validation of a Nanosecond Time-Resolved Micro-Spectrophotometer Design Using Photoactive Yellow Protein and α-Phycoerythrocyanin
Next Article in Special Issue
Achyranthes bidentata Polypeptides Reduces Oxidative Stress and Exerts Protective Effects against Myocardial Ischemic/Reperfusion Injury in Rats
Previous Article in Journal
A Facile and Efficient Synthesis of Diaryl Amines or Ethers under Microwave Irradiation at Presence of KF/Al2O3 without Solvent and Their Anti-Fungal Biological Activities against Six Phytopathogens
Previous Article in Special Issue
Redox Signaling Is an Early Event in the Pathogenesis of Renovascular Hypertension
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2013, 14(9), 18861-18880; doi:10.3390/ijms140918861

Oleic Acid Increases Synthesis and Secretion of VEGF in Rat Vascular Smooth Muscle Cells: Role of Oxidative Stress and Impairment in Obesity

Internal Medicine and Metabolic Disease Unit, Department of Clinical and Biological Sciences of the University of Turin, San Luigi Gonzaga Hospital, Orbassano (Turin) 10043, Italy
This author deceased on 9 April 2011.
*
Author to whom correspondence should be addressed.
Received: 31 May 2013 / Revised: 5 August 2013 / Accepted: 5 September 2013 / Published: 13 September 2013
(This article belongs to the Special Issue Oxidative Stress in Cardiovascular Disease)
View Full-Text   |   Download PDF [1376 KB, uploaded 19 June 2014]   |  

Abstract

Obesity is characterized by poor collateral vessel formation, a process involving vascular endothelial growth factor (VEGF) action on vascular smooth muscle cells (VSMC). Free fatty acids are involved in the pathogenesis of obesity vascular complications, and we have aimed to clarify whether oleic acid (OA) enhances VEGF synthesis/secretion in VSMC, and whether this effect is impaired in obesity. In cultured aortic VSMC from lean and obese Zucker rats (LZR and OZR, respectively) we measured the influence of OA on VEGF-A synthesis/secretion, signaling molecules and reactive oxygen species (ROS). In VSMC from LZR we found the following: (a) OA increases VEGF-A synthesis/secretion by a mechanism blunted by inhibitors of Akt, mTOR, ERK-1/2, PKC-beta, NADPH-oxidase and mitochondrial electron transport chain complex; (b) OA activates the above mentioned signaling pathways and increases ROS; (c) OA-induced activation of PKC-beta enhances oxidative stress, which activates signaling pathways responsible for the increased VEGF synthesis/secretion. In VSMC from OZR, which present enhanced baseline oxidative stress, the above mentioned actions of OA on VEGF-A, signaling pathways and ROS are impaired: this impairment is reproduced in VSMC from LZR by incubation with hydrogen peroxide. Thus, in OZR chronically elevated oxidative stress causes a resistance to the action on VEGF that OA exerts in LZR by increasing ROS.
Keywords: obesity; vascular smooth muscle cells; vascular endothelial growth factor; oleic acid; oxidative stress; protein kinase C; Zucker rats; mitogen activated protein kinase; phosphatidylinositol 3-kinase obesity; vascular smooth muscle cells; vascular endothelial growth factor; oleic acid; oxidative stress; protein kinase C; Zucker rats; mitogen activated protein kinase; phosphatidylinositol 3-kinase
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Doronzo, G.; Viretto, M.; Barale, C.; Russo, I.; Mattiello, L.; Anfossi, G.; Trovati, M. Oleic Acid Increases Synthesis and Secretion of VEGF in Rat Vascular Smooth Muscle Cells: Role of Oxidative Stress and Impairment in Obesity. Int. J. Mol. Sci. 2013, 14, 18861-18880.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top