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Int. J. Mol. Sci. 2013, 14(7), 15179-15198; doi:10.3390/ijms140715179

Characterization of a Gene Encoding Clathrin Heavy Chain in Maize Up-Regulated by Salicylic Acid, Abscisic Acid and High Boron Supply

College of Life Sciences, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
These authors contributed equally to this work.
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Received: 16 May 2013 / Revised: 1 July 2013 / Accepted: 16 July 2013 / Published: 22 July 2013
(This article belongs to the Special Issue Regulation of Membrane Trafficking and Its Potential Implications)
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Abstract

Clathrin, a three-legged triskelion composed of three clathrin heavy chains (CHCs) and three light chains (CLCs), plays a critical role in clathrin-mediated endocytosis (CME) in eukaryotic cells. In this study, the genes ZmCHC1 and ZmCHC2 encoding clathrin heavy chain in maize were cloned and characterized for the first time in monocots. ZmCHC1 encodes a 1693-amino acid-protein including 29 exons and 28 introns, and ZmCHC2 encodes a 1746-amino acid-protein including 28 exons and 27 introns. The high similarities of gene structure, protein sequences and 3D models among ZmCHC1, and Arabidopsis AtCHC1 and AtCHC2 suggest their similar functions in CME. ZmCHC1 gene is predominantly expressed in maize roots instead of ubiquitous expression of ZmCHC2. Consistent with a typical predicted salicylic acid (SA)-responsive element and four predicted ABA-responsive elements (ABREs) in the promoter sequence of ZmCHC1, the expression of ZmCHC1 instead of ZmCHC2 in maize roots is significantly up-regulated by SA or ABA, suggesting that ZmCHC1 gene may be involved in the SA signaling pathway in maize defense responses. The expressions of ZmCHC1 and ZmCHC2 genes in maize are down-regulated by azide or cold treatment, further revealing the energy requirement of CME and suggesting that CME in plants is sensitive to low temperatures.
Keywords: clathrin heavy chain; gene cloning and expression; salicylic acid and abiotic stresses; maize (Zea mays L.) clathrin heavy chain; gene cloning and expression; salicylic acid and abiotic stresses; maize (Zea mays L.)
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Zeng, M.-H.; Liu, S.-H.; Yang, M.-X.; Zhang, Y.-J.; Liang, J.-Y.; Wan, X.-R.; Liang, H. Characterization of a Gene Encoding Clathrin Heavy Chain in Maize Up-Regulated by Salicylic Acid, Abscisic Acid and High Boron Supply. Int. J. Mol. Sci. 2013, 14, 15179-15198.

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