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Int. J. Mol. Sci. 2013, 14(6), 11259-11276; doi:10.3390/ijms140611259
Article

New Evidence for Cross Talk between Melatonin and Mitochondria Mediated by a Circadian-Compatible Interaction with Nitric Oxide

1,2,* , 1
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1
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2
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Received: 22 March 2013 / Revised: 16 May 2013 / Accepted: 16 May 2013 / Published: 28 May 2013
(This article belongs to the Special Issue Advances in the Research of Melatonin)
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Abstract

Extending our previous observations, we have shown on HaCat cells that melatonin, at ~10−9 M concentration, transiently raises not only the expression of the neuronal nitric oxide synthase (nNOS) mRNA, but also the nNOS protein synthesis and the nitric oxide oxidation products, nitrite and nitrate. Interestingly, from the cell bioenergetic point of view, the activated NO-related chemistry induces a mild decrease of the oxidative phosphorylation (OXPHOS) efficiency, paralleled by a depression of the mitochondrial membrane potential. The OXPHOS depression is apparently balanced by glycolysis. The mitochondrial effects described have been detected only at nanomolar concentration of melatonin and within a time window of a few hours’ incubation; both findings compatible with the melatonin circadian cycle.
Keywords: nitric oxide; cell bioenergetics; respiratory chain; circadian rhythm; cell culture; Warburg effect; reactive oxygen; nitrogen species nitric oxide; cell bioenergetics; respiratory chain; circadian rhythm; cell culture; Warburg effect; reactive oxygen; nitrogen species
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Sarti, P.; Magnifico, M.C.; Altieri, F.; Mastronicola, D.; Arese, M. New Evidence for Cross Talk between Melatonin and Mitochondria Mediated by a Circadian-Compatible Interaction with Nitric Oxide. Int. J. Mol. Sci. 2013, 14, 11259-11276.

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