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Current Status and Future Perspectives of Mass Spectrometry Imaging
Inhalation Toxicology Laboratory, Environmental Health Science and Research Bureau, Healthy Environments and Consumer Safety Branch, Health Canada, Ottawa, ON K1A0K9, Canada
Analytical Biochemistry and Proteomics Laboratory, Environmental Health Science and Research Bureau, Healthy Environments and Consumer Safety Branch, Health Canada, Ottawa, ON K1A0K9, Canada
* Author to whom correspondence should be addressed.
Received: 12 April 2013; in revised form: 9 May 2013 / Accepted: 13 May 2013 / Published: 28 May 2013
Abstract: Mass spectrometry imaging is employed for mapping proteins, lipids and metabolites in biological tissues in a morphological context. Although initially developed as a tool for biomarker discovery by imaging the distribution of protein/peptide in tissue sections, the high sensitivity and molecular specificity of this technique have enabled its application to biomolecules, other than proteins, even in cells, latent finger prints and whole organisms. Relatively simple, with no requirement for labelling, homogenization, extraction or reconstitution, the technique has found a variety of applications in molecular biology, pathology, pharmacology and toxicology. By discriminating the spatial distribution of biomolecules in serial sections of tissues, biomarkers of lesions and the biological responses to stressors or diseases can be better understood in the context of structure and function. In this review, we have discussed the advances in the different aspects of mass spectrometry imaging processes, application towards different disciplines and relevance to the field of toxicology.
Keywords: MALDI imaging; proteins; lipids; metabolites; mass spectrometry; tissues; matrix; toxicology
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MDPI and ACS Style
Nimesh, S.; Mohottalage, S.; Vincent, R.; Kumarathasan, P. Current Status and Future Perspectives of Mass Spectrometry Imaging. Int. J. Mol. Sci. 2013, 14, 11277-11301.
Nimesh S, Mohottalage S, Vincent R, Kumarathasan P. Current Status and Future Perspectives of Mass Spectrometry Imaging. International Journal of Molecular Sciences. 2013; 14(6):11277-11301.
Nimesh, Surendra; Mohottalage, Susantha; Vincent, Renaud; Kumarathasan, Prem. 2013. "Current Status and Future Perspectives of Mass Spectrometry Imaging." Int. J. Mol. Sci. 14, no. 6: 11277-11301.