Int. J. Mol. Sci. 2013, 14(1), 205-225; doi:10.3390/ijms14010205
Article

Expression and Functional Studies on the Noncoding RNA, PRINS

1,†email, 1,†,* email, 1email, 2email, 1email, 1,3email, 1,3email, 1email and 3,4email
Received: 5 November 2012; in revised form: 30 November 2012 / Accepted: 10 December 2012 / Published: 21 December 2012
(This article belongs to the Special Issue Non-Coding RNAs 2012)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: PRINS, a noncoding RNA identified earlier by our research group, contributes to psoriasis susceptibility and cellular stress response. We have now studied the cellular and histological distribution of PRINS by using in situ hybridization and demonstrated variable expressions in different human tissues and a consistent staining pattern in epidermal keratinocytes and in vitro cultured keratinocytes. To identify the cellular function(s) of PRINS, we searched for a direct interacting partner(s) of this stress-induced molecule. In HaCaT and NHEK cell lysates, the protein proved to be nucleophosmin (NPM) protein as a potential physical interactor with PRINS. Immunohistochemical experiments revealed an elevated expression of NPM in the dividing cells of the basal layers of psoriatic involved skin samples as compared with healthy and psoriatic uninvolved samples. Others have previously shown that NPM is a ubiquitously expressed nucleolar phosphoprotein which shuttles to the nucleoplasm after UV-B irradiation in fibroblasts and cancer cells. We detected a similar translocation of NPM in UV-B-irradiated cultured keratinocytes. The gene-specific silencing of PRINS resulted in the retention of NPM in the nucleolus of UV-B-irradiated keratinocytes; suggesting that PRINS may play a role in the NPM-mediated cellular stress response in the skin.
Keywords: noncoding RNA; nucleophosmin; keratinocyte proliferation; psoriasis; UV-B irradiation
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MDPI and ACS Style

Szegedi, K.; Göblös, A.; Bacsa, S.; Antal, M.; Németh, I.B.; Bata-Csörgő, Z.; Kemény, L.; Dobozy, A.; Széll, M. Expression and Functional Studies on the Noncoding RNA, PRINS. Int. J. Mol. Sci. 2013, 14, 205-225.

AMA Style

Szegedi K, Göblös A, Bacsa S, Antal M, Németh IB, Bata-Csörgő Z, Kemény L, Dobozy A, Széll M. Expression and Functional Studies on the Noncoding RNA, PRINS. International Journal of Molecular Sciences. 2013; 14(1):205-225.

Chicago/Turabian Style

Szegedi, Krisztina; Göblös, Anikó; Bacsa, Sarolta; Antal, Mária; Németh, István B.; Bata-Csörgő, Zsuzsanna; Kemény, Lajos; Dobozy, Attila; Széll, Márta. 2013. "Expression and Functional Studies on the Noncoding RNA, PRINS." Int. J. Mol. Sci. 14, no. 1: 205-225.

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