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Expression and Functional Studies on the Noncoding RNA, PRINS
Department of Dermatology and Allergology, University of Szeged, Korányi fasor 6, H-6720 Szeged, Hungary
Institute of Plant Biology, Biological Research Center of the Hungarian Academy of Sciences, Temesvári krt 62, H-6726 Szeged, Hungary
Dermatological Research Group of the Hungarian Academy of Sciences, University of Szeged, Korányi fasor 6, H-6720 Szeged, Hungary
Department of Medical Genetics, University of Szeged, Somogyi u. 4, H-6720 Szeged, Hungary
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 5 November 2012; in revised form: 30 November 2012 / Accepted: 10 December 2012 / Published: 21 December 2012
Abstract: PRINS, a noncoding RNA identified earlier by our research group, contributes to psoriasis susceptibility and cellular stress response. We have now studied the cellular and histological distribution of PRINS by using in situ hybridization and demonstrated variable expressions in different human tissues and a consistent staining pattern in epidermal keratinocytes and in vitro cultured keratinocytes. To identify the cellular function(s) of PRINS, we searched for a direct interacting partner(s) of this stress-induced molecule. In HaCaT and NHEK cell lysates, the protein proved to be nucleophosmin (NPM) protein as a potential physical interactor with PRINS. Immunohistochemical experiments revealed an elevated expression of NPM in the dividing cells of the basal layers of psoriatic involved skin samples as compared with healthy and psoriatic uninvolved samples. Others have previously shown that NPM is a ubiquitously expressed nucleolar phosphoprotein which shuttles to the nucleoplasm after UV-B irradiation in fibroblasts and cancer cells. We detected a similar translocation of NPM in UV-B-irradiated cultured keratinocytes. The gene-specific silencing of PRINS resulted in the retention of NPM in the nucleolus of UV-B-irradiated keratinocytes; suggesting that PRINS may play a role in the NPM-mediated cellular stress response in the skin.
Keywords: noncoding RNA; nucleophosmin; keratinocyte proliferation; psoriasis; UV-B irradiation
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Szegedi, K.; Göblös, A.; Bacsa, S.; Antal, M.; Németh, I.B.; Bata-Csörgő, Z.; Kemény, L.; Dobozy, A.; Széll, M. Expression and Functional Studies on the Noncoding RNA, PRINS. Int. J. Mol. Sci. 2013, 14, 205-225.
Szegedi K, Göblös A, Bacsa S, Antal M, Németh IB, Bata-Csörgő Z, Kemény L, Dobozy A, Széll M. Expression and Functional Studies on the Noncoding RNA, PRINS. International Journal of Molecular Sciences. 2013; 14(1):205-225.
Szegedi, Krisztina; Göblös, Anikó; Bacsa, Sarolta; Antal, Mária; Németh, István B.; Bata-Csörgő, Zsuzsanna; Kemény, Lajos; Dobozy, Attila; Széll, Márta. 2013. "Expression and Functional Studies on the Noncoding RNA, PRINS." Int. J. Mol. Sci. 14, no. 1: 205-225.