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A correction was published on 3 May 2013, see Int. J. Mol. Sci. 2013, 14(5), 9685.

Int. J. Mol. Sci. 2013, 14(1), 378-393; doi:10.3390/ijms14010378
Article

Exploration of the Role of the Non-Coding RNA SbrE in L. monocytogenes Stress Response

1
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 and 1,*
Received: 5 November 2012; in revised form: 11 December 2012 / Accepted: 14 December 2012 / Published: 24 December 2012
(This article belongs to the Special Issue Non-Coding RNAs 2012)
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Abstract: SbrE is a ncRNA in Listeria monocytogenes, reported to be up-regulated by the alternative sigma factor σB. Initial quantitative RT-PCR (qRT-PCR) experiments on parent strains and isogenic ΔsigB strains demonstrated σB-dependent expression of SbrE across the four L. monocytogenes lineages and in L. innocua. Microarray and proteomics (MDLC/MS/MS with iTRAQ labeling) experiments with the L. monocytogenes parent strain and an isogenic ΔsbrE strain identified a single gene (lmo0636) and two proteins (Lmo0637 and Lmo2094) that showed lower expression levels in the ΔsbrE strain. qRT-PCR demonstrated an increase in SbrE transcript levels in stationary phase L. monocytogenes and in bacteria exposed to oxidative stress (mean log2 transcript levels 7.68 ± 0.57 and 1.70 ± 0.71 greater than in mid-log phase cells, respectively). However, no significant differences in growth or survival between the parent strain and ΔsbrE strain were confirmed under a variety of environmental stress conditions tested. Our data suggest that σB-dependent transcription of SbrE represents a conserved mechanism that contributes, across Listeria species, to fine-tuning of gene expression under specific environmental conditions that remain to be defined.
Keywords: non-coding RNA; Listeria monocytogenes; sigma B non-coding RNA; Listeria monocytogenes; sigma B
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Mujahid, S.; Bergholz, T.M.; Oliver, H.F.; Boor, K.J.; Wiedmann, M. Exploration of the Role of the Non-Coding RNA SbrE in L. monocytogenes Stress Response. Int. J. Mol. Sci. 2013, 14, 378-393.

AMA Style

Mujahid S, Bergholz TM, Oliver HF, Boor KJ, Wiedmann M. Exploration of the Role of the Non-Coding RNA SbrE in L. monocytogenes Stress Response. International Journal of Molecular Sciences. 2013; 14(1):378-393.

Chicago/Turabian Style

Mujahid, Sana; Bergholz, Teresa M.; Oliver, Haley F.; Boor, Kathryn J.; Wiedmann, Martin. 2013. "Exploration of the Role of the Non-Coding RNA SbrE in L. monocytogenes Stress Response." Int. J. Mol. Sci. 14, no. 1: 378-393.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert