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• Mujahid, S
• Bergholz, TM.
• Oliver, HF.
• Boor, KJ.
• Wiedmann, M
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• Mujahid, S
• Bergholz, TM.
• Oliver, HF.
• Boor, KJ.
• Wiedmann, M
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• Mujahid, S
• Bergholz, TM.
• Oliver, HF.
• Boor, KJ.
• Wiedmann, M

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Int. J. Mol. Sci. 2013, 14(1), 378-393; doi:10.3390/ijms14010378

Article

Exploration of the Role of the Non-Coding RNA SbrE in L. monocytogenes Stress Response

Sana Mujahid ¹ , Teresa M. Bergholz ¹ , Haley F. Oliver ^1,2 , Kathryn J. Boor ¹  and Martin Wiedmann ^1,*

¹ Department of Food Science, Cornell University, Ithaca, NY 14853, USA ² Department of Food Science, Purdue University, West Lafayette, IN 47907, USA

* Author to whom correspondence should be addressed.

Received: 5 November 2012; in revised form: 11 December 2012 / Accepted: 14 December 2012 / Published: 24 December 2012

(This article belongs to the Special Issue Non-Coding RNAs 2012)

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Abstract: SbrE is a ncRNA in Listeria monocytogenes, reported to be up-regulated by the alternative sigma factor σ^B. Initial quantitative RT-PCR (qRT-PCR) experiments on parent strains and isogenic ΔsigB strains demonstrated σ^B-dependent expression of SbrE across the four L. monocytogenes lineages and in L. innocua. Microarray and proteomics (MDLC/MS/MS with iTRAQ labeling) experiments with the L. monocytogenes parent strain and an isogenic ΔsbrE strain identified a single gene (lmo0636) and two proteins (Lmo0637 and Lmo2094) that showed lower expression levels in the ΔsbrE strain. qRT-PCR demonstrated an increase in SbrE transcript levels in stationary phase L. monocytogenes and in bacteria exposed to oxidative stress (mean log2 transcript levels 7.68 ± 0.57 and 1.70 ± 0.71 greater than in mid-log phase cells, respectively). However, no significant differences in growth or survival between the parent strain and ΔsbrE strain were confirmed under a variety of environmental stress conditions tested. Our data suggest that σ^B-dependent transcription of SbrE represents a conserved mechanism that contributes, across Listeria species, to fine-tuning of gene expression under specific environmental conditions that remain to be defined.

Keywords: non-coding RNA; Listeria monocytogenes; sigma B

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