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Exploration of the Role of the Non-Coding RNA SbrE in L. monocytogenes Stress Response
Department of Food Science, Cornell University, Ithaca, NY 14853, USA
Department of Food Science, Purdue University, West Lafayette, IN 47907, USA
* Author to whom correspondence should be addressed.
Received: 5 November 2012; in revised form: 11 December 2012 / Accepted: 14 December 2012 / Published: 24 December 2012
Abstract: SbrE is a ncRNA in Listeria monocytogenes, reported to be up-regulated by the alternative sigma factor σB. Initial quantitative RT-PCR (qRT-PCR) experiments on parent strains and isogenic ΔsigB strains demonstrated σB-dependent expression of SbrE across the four L. monocytogenes lineages and in L. innocua. Microarray and proteomics (MDLC/MS/MS with iTRAQ labeling) experiments with the L. monocytogenes parent strain and an isogenic ΔsbrE strain identified a single gene (lmo0636) and two proteins (Lmo0637 and Lmo2094) that showed lower expression levels in the ΔsbrE strain. qRT-PCR demonstrated an increase in SbrE transcript levels in stationary phase L. monocytogenes and in bacteria exposed to oxidative stress (mean log2 transcript levels 7.68 ± 0.57 and 1.70 ± 0.71 greater than in mid-log phase cells, respectively). However, no significant differences in growth or survival between the parent strain and ΔsbrE strain were confirmed under a variety of environmental stress conditions tested. Our data suggest that σB-dependent transcription of SbrE represents a conserved mechanism that contributes, across Listeria species, to fine-tuning of gene expression under specific environmental conditions that remain to be defined.
Keywords: non-coding RNA; Listeria monocytogenes; sigma B
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Mujahid, S.; Bergholz, T.M.; Oliver, H.F.; Boor, K.J.; Wiedmann, M. Exploration of the Role of the Non-Coding RNA SbrE in L. monocytogenes Stress Response. Int. J. Mol. Sci. 2013, 14, 378-393.
Mujahid S, Bergholz TM, Oliver HF, Boor KJ, Wiedmann M. Exploration of the Role of the Non-Coding RNA SbrE in L. monocytogenes Stress Response. International Journal of Molecular Sciences. 2013; 14(1):378-393.
Mujahid, Sana; Bergholz, Teresa M.; Oliver, Haley F.; Boor, Kathryn J.; Wiedmann, Martin. 2013. "Exploration of the Role of the Non-Coding RNA SbrE in L. monocytogenes Stress Response." Int. J. Mol. Sci. 14, no. 1: 378-393.