Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Int. J. Mol. Sci. 2012, 13(12), 16718-16736; doi:10.3390/ijms131216718
Article

UVA Irradiation of Dysplastic Keratinocytes: Oxidative Damage versus Antioxidant Defense

1,†
, 2,†
, 2,†
, 2,3
, 2
, 2
, 2
, 1
 and 2,3,*
Received: 5 September 2012; in revised form: 13 November 2012 / Accepted: 29 November 2012 / Published: 6 December 2012
(This article belongs to the Special Issue UV-Induced Cell Death 2012)
View Full-Text   |   Download PDF [1050 KB, uploaded 19 June 2014]
Abstract: UVA affects epidermal cell physiology in a complex manner, but the harmful effects have been studied mainly in terms of DNA damage, mutagenesis and carcinogenesis. We investigated UVA effects on membrane integrity and antioxidant defense of dysplastic keratinocytes after one and two hours of irradiation, both immediately after exposure, and 24 h post-irradiation. To determine the UVA oxidative stress on cell membrane, lipid peroxidation was correlated with changes in fatty acid levels. Membrane permeability and integrity were assessed by propidium iodide staining and lactate dehydrogenase release. The effects on keratinocyte antioxidant protection were investigated in terms of catalase activity and expression. Lipid peroxidation increased in an exposure time-dependent manner. UVA exposure decreased the level of polyunsaturated fatty acids, which gradually returned to its initial value. Lactate dehydrogenase release showed a dramatic loss in membrane integrity after 2 h minimum of exposure. The cell ability to restore membrane permeability was noted at 24 h post-irradiation (for one hour exposure). Catalase activity decreased in an exposure time-dependent manner. UVA-irradiated dysplastic keratinocytes developed mechanisms leading to cell protection and survival, following a non-lethal exposure. The surviving cells gained an increased resistance to apoptosis, suggesting that their pre-malignant status harbors an abnormal ability to control their fate.
Keywords: dysplastic keratinocyte; UVA; oxidative stress; membrane integrity; lipid peroxidation; LDH release; catalase dysplastic keratinocyte; UVA; oxidative stress; membrane integrity; lipid peroxidation; LDH release; catalase
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Nechifor, M.T.; Niculiţe, C.M.; Urs, A.O.; Regalia, T.; Mocanu, M.; Popescu, A.; Manda, G.; Dinu, D.; Leabu, M. UVA Irradiation of Dysplastic Keratinocytes: Oxidative Damage versus Antioxidant Defense. Int. J. Mol. Sci. 2012, 13, 16718-16736.

AMA Style

Nechifor MT, Niculiţe CM, Urs AO, Regalia T, Mocanu M, Popescu A, Manda G, Dinu D, Leabu M. UVA Irradiation of Dysplastic Keratinocytes: Oxidative Damage versus Antioxidant Defense. International Journal of Molecular Sciences. 2012; 13(12):16718-16736.

Chicago/Turabian Style

Nechifor, Marina T.; Niculiţe, Cristina M.; Urs, Andreea O.; Regalia, Teodor; Mocanu, Mihaela; Popescu, Alexandra; Manda, Gina; Dinu, Diana; Leabu, Mircea. 2012. "UVA Irradiation of Dysplastic Keratinocytes: Oxidative Damage versus Antioxidant Defense." Int. J. Mol. Sci. 13, no. 12: 16718-16736.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert