Int. J. Mol. Sci. 2012, 13(12), 16444-16456; doi:10.3390/ijms131216444
Article

A Comparative Analysis of the Photo-Protective Effects of Soy Isoflavones in Their Aglycone and Glucoside Forms

Received: 19 September 2012; in revised form: 24 November 2012 / Accepted: 29 November 2012 / Published: 4 December 2012
(This article belongs to the Special Issue UV-Induced Cell Death 2012)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Isoflavones exist in nature predominantly as glucosides such as daidzin or genistin and are rarely found in their corresponding aglycone forms daidzein and genistein. The metabolism and absorption of isoflavones ingested with food is well documented, but little is known about their use as topical photo-protective agents. The aim of this study was to investigate in a comparative analysis the photo-protective effects of isoflavones in both their aglycone and glucoside forms. In human skin fibroblasts irradiated with 60 mJ/cm2 ultraviolet B (UVB), we measured the expression levels of COX-2 and Gadd45, which are involved in inflammation and DNA repair, respectively. We also determined the cellular response to UVB-induced DNA damage using the comet assay. Our findings suggest that both the isoflavone glucosides at a specific concentration and combination with an aglycone mixture exerted an anti-inflammatory and photo-protective effect that prevented 41% and 71% of UVB-induced DNA damage, respectively. The advantages of using either isoflavone glucosides or an aglycone mixture in applications in the field of dermatology will depend on their properties and their different potential uses.
Keywords: isoflavones; cyclooxygenase-2; DNA-damage inducible gene; UVB; DNA damage; anti-inflammatory; photo-protective
PDF Full-text Download PDF Full-Text [1035 KB, uploaded 19 June 2014 04:34 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Iovine, B.; Iannella, M.L.; Gasparri, F.; Giannini, V.; Monfrecola, G.; Bevilacqua, M.A. A Comparative Analysis of the Photo-Protective Effects of Soy Isoflavones in Their Aglycone and Glucoside Forms. Int. J. Mol. Sci. 2012, 13, 16444-16456.

AMA Style

Iovine B, Iannella ML, Gasparri F, Giannini V, Monfrecola G, Bevilacqua MA. A Comparative Analysis of the Photo-Protective Effects of Soy Isoflavones in Their Aglycone and Glucoside Forms. International Journal of Molecular Sciences. 2012; 13(12):16444-16456.

Chicago/Turabian Style

Iovine, Barbara; Iannella, Maria L.; Gasparri, Franco; Giannini, Valentina; Monfrecola, Giuseppe; Bevilacqua, Maria A. 2012. "A Comparative Analysis of the Photo-Protective Effects of Soy Isoflavones in Their Aglycone and Glucoside Forms." Int. J. Mol. Sci. 13, no. 12: 16444-16456.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert