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Inhibitor of Apoptosis Protein-Like Protein-2 as a Novel Serological Biomarker for Breast Cancer
Department of Biochemistry & Molecular Biology, College of Medical Science, Jishou University, Jishou 416000, China
Department of Biochemistry & Molecular Biology, Gannan Medical University, Ganzhou 341000, China
Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Bio-Safety Science & Technology, Hunan Agricultural University, Changsha 410128, China
Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118, China
Clinical Laboratory, the First Affiliated Hospital, Gannan Medical University, Ganzhou 341000, China
These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 28 September 2012; in revised form: 22 November 2012 / Accepted: 30 November 2012 / Published: 7 December 2012
Abstract: Inhibitor of apoptosis protein-like protein-2 (ILP-2) has only been detected in the testis and in lymphoblastoid cells. Although previous studies have not reported the presence of ILP-2 in breast cancer tissues, this study indicates the presence of ILP-2 in breast cancer serum samples. To validate whether ILP-2 is a novel serological biomarker for breast cancer, we conducted two-dimensional gel electrophoresis (2DE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry analysis on 400 breast cancer serum samples and 40 non-cancer serum samples (i.e., healthy controls). We then performed a Western blot analysis of 10 breast cancer serum samples and 10 non-cancer serum samples. Finally, we analyzed 35 serum samples from healthy controls or subjects with breast cancer, other types of cancer, galactophore hyperplasia or breast cancer post-surgery by using 2DE and enzyme-linked immunosorbent assay. Our results indicate that ILP-2 is a novel breast cancer biomarker in the peripheral blood.
Keywords: ILP-2; breast cancer; serological biomarker
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Xiang, M.; Zhou, W.; Gao, D.; Fang, X.; Liu, Q. Inhibitor of Apoptosis Protein-Like Protein-2 as a Novel Serological Biomarker for Breast Cancer. Int. J. Mol. Sci. 2012, 13, 16737-16750.
Xiang M, Zhou W, Gao D, Fang X, Liu Q. Inhibitor of Apoptosis Protein-Like Protein-2 as a Novel Serological Biomarker for Breast Cancer. International Journal of Molecular Sciences. 2012; 13(12):16737-16750.
Xiang, Mingjun; Zhou, Wei; Gao, Dandan; Fang, Xiansong; Liu, Qian. 2012. "Inhibitor of Apoptosis Protein-Like Protein-2 as a Novel Serological Biomarker for Breast Cancer." Int. J. Mol. Sci. 13, no. 12: 16737-16750.