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Molecules 2017, 22(8), 1310; doi:10.3390/molecules22081310

Isolation and Purification of Three Ecdysteroids from the Stems of Diploclisia glaucescens by High-Speed Countercurrent Chromatography and Their Anti-Inflammatory Activities In Vitro

1,2
,
2
,
1,2,* , 2
and
3,*
1
School of Biological Science and Technology, University of Jinan, Jinan 250022, China
2
Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Jinan 250014, China
3
State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
*
Authors to whom correspondence should be addressed.
Received: 25 July 2017 / Accepted: 30 July 2017 / Published: 7 August 2017
(This article belongs to the Special Issue Anti-inflammatory Agents)
View Full-Text   |   Download PDF [956 KB, uploaded 7 August 2017]   |  

Abstract

High-speed counter-current chromatography was used to separate and purify ecdysteroids for the first time from the stems of Diploclisia glaucescens using a two-phase solvent system composed of ethyl acetate–n-butanol–ethanol–water (3:0.2:0.8:3, v/v). Three ecdysteroids were obtained from 260 mg of ethyl acetate extract of the residue obtained after evaporation of the crude ethanolicextractof D. glaucescens in one-step separation, which were identified as paristerone (I, 30.5 mg), ecdysterone (II, 7.2 mg), and capitasterone (III, 8.1 mg) by electrospray ionization mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR). Their anti-inflammatory activities were evaluated by measuring the inhibitory ratios of β-glucuronidase release in rat polymorphonuclear leukocytes (PMNs) induced by platelet-activating factor. Compounds IIII showed significant anti-inflammatory activities with IC50-values ranging from 1.51 to 11.68 μM, respectively. View Full-Text
Keywords: Diploclisia glaucescens; ecdysteroids; high-speed counter-current chromatography; anti-inflammatory activities Diploclisia glaucescens; ecdysteroids; high-speed counter-current chromatography; anti-inflammatory activities
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Fang, L.; Li, J.; Zhou, J.; Wang, X.; Guo, L. Isolation and Purification of Three Ecdysteroids from the Stems of Diploclisia glaucescens by High-Speed Countercurrent Chromatography and Their Anti-Inflammatory Activities In Vitro. Molecules 2017, 22, 1310.

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