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Molecules 2017, 22(8), 1286; doi:10.3390/molecules22081286

Optimization of the Ultrasound-Assisted Extraction of Phenolic Compounds from Brosimum alicastrum Leaves and the Evaluation of Their Radical-Scavenging Activity

Experimental Unit, University Marist of Mérida, Periférico Norte Tablaje Catastral 13941, Carretera Mérida-Progreso, 97300 Mérida, Yucatán, Mexico
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Received: 17 July 2017 / Revised: 29 July 2017 / Accepted: 31 July 2017 / Published: 7 August 2017
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Abstract

In order to maximize the yield of the total phenolic content (TPC) and total monomeric anthocyanin (TMA) from Brosimum alicastrum leaf and to study the radical-scavenging activity, a three-level three-factor Box–Behnken design (BBD) was used to determine the optimal points for ultrasound-assisted extraction (UAE). In this study, we analyzed the extraction time (10, 20, and 30 min), temperature (28, 30, and 32 °C), and probe sonication power (40%, 28 W/cm2; 60%, 51 W/cm2; and 80%, 74 W/cm2). Analysis of variance (ANOVA) indicated that the sonication power plays a significant role in the extraction of phenolic compounds. An increase in time and temperature resulted in a decrease in the yield, in particular, of the TMA group. DPPH was found to be a better indicator of radical-scavenging activity than ABTS. The predicted TPC and TMA optimum levels (45.18 mg GAE/g and 15.16 mg CyE/100 g) were obtained at 28 °C, 80%, and 20–10 min. DPPH obtained a maximum value (67.27 μmol TE/g) under same optimization conditions. The RSM confirmed that TPC and TMA enhanced the antioxidant activity when subjected to low temperature (28 °C), extraction time less than 20 min, and higher sonication power (74 W/cm2), and hence achieving the better DPPH scavenging activity. View Full-Text
Keywords: Brosimum alicastrum leaf; ultrasound assisted extraction; response surface methodology; total phenolic content; monomeric anthocyanin; radical-scavenging activity Brosimum alicastrum leaf; ultrasound assisted extraction; response surface methodology; total phenolic content; monomeric anthocyanin; radical-scavenging activity
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Gullian Klanian, M.; Terrats Preciat, M. Optimization of the Ultrasound-Assisted Extraction of Phenolic Compounds from Brosimum alicastrum Leaves and the Evaluation of Their Radical-Scavenging Activity. Molecules 2017, 22, 1286.

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