Development of HA/Ag-NPs Composite Coating from Green Process for Hip Applications
AbstractIn the present study, biological hydroxyapatite (HA) was obtained from bovine bones through a thermal process. A total of 0% and 1% of silver nanoparticles (Ag-NPs) synthesized from Opuntia ficus (nopal) were added to the biological hydroxyapatite coatings using an atmospheric plasma spray (APS) on a Ti6Al4V substrate. Following this, its antimicrobial efficiency was evaluated against the following bacterial strains: Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. This was conducted according to the Japanese Industrial Standard (JIS) Z2801:2000 “Antimicrobial Product-Test for Antimicrobial Activity and Efficacy”. Scanning electron microscopy (SEM) showed that the silver nanoparticles (Ag-NPs) were evenly distributed on the coating surface. Energy dispersive X-ray spectroscopy (EDX) shows that apatite deposition occurs on a daily basis, maintaining a Ca/P rate between 2.12 and 1.45. Biocompatibility properties were evaluated with osteoblast-like cells (MC3T3-E1) by single-cell gel electrophoresis assay and Tali image cytometry. View Full-Text
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Lozoya-Rodríguez, D.A.; de Lima, R.; Fraceto, L.F.; Ledezma Pérez, A.; Bazaldua Domínguez, M.; Gómez Batres, R.; Reyes Rojas, A.; Orozco Carmona, V. Development of HA/Ag-NPs Composite Coating from Green Process for Hip Applications. Molecules 2017, 22, 1291.
Lozoya-Rodríguez DA, de Lima R, Fraceto LF, Ledezma Pérez A, Bazaldua Domínguez M, Gómez Batres R, Reyes Rojas A, Orozco Carmona V. Development of HA/Ag-NPs Composite Coating from Green Process for Hip Applications. Molecules. 2017; 22(8):1291.Chicago/Turabian Style
Lozoya-Rodríguez, Denisse A.; de Lima, Renata; Fraceto, Leonardo F.; Ledezma Pérez, Antonio; Bazaldua Domínguez, Mercedes; Gómez Batres, Roberto; Reyes Rojas, Armando; Orozco Carmona, Víctor. 2017. "Development of HA/Ag-NPs Composite Coating from Green Process for Hip Applications." Molecules 22, no. 8: 1291.
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