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Molecules 2017, 22(7), 1044; doi:10.3390/molecules22071044

Osteoprotective Effect of Radix Scutellariae in Female Hindlimb-Suspended Sprague-Dawley Rats and the Osteogenic Differentiation Effect of Its Major Constituent

1
Research Institute of Atherosclerotic Disease, College of Clinical Medicine, Xi’an Medical University, No.1Xinwang Road, Xi’an 710021, China
2
Laboratory Animal Center, Xi′an Jiaotong University Health Science Center, Xi’an Jiaotong University, Xi’an 710061, China
3
Key Laboratory for Space Biosciences & Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyixi Road, Xi’an 710072, China
Current address: Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Department of Public Health, College of Clinical Medicine, Xi’an Medical University, Xi’an 710021, China.
*
Author to whom correspondence should be addressed.
Academic Editor: Hala Gali-Muhtasib
Received: 31 March 2017 / Revised: 23 May 2017 / Accepted: 15 June 2017 / Published: 3 July 2017
View Full-Text   |   Download PDF [1529 KB, uploaded 11 July 2017]   |  

Abstract

A number of medicinal herbs have demonstrated therapeutic effects for the prevention and treatment of disuse-induced osteoporosis. As a common ingredient in proprietary traditional Chinese medicines, the anti-osteoporosis effects of Radix Scutellariae extract (RSE, 50 mg/kg/day) were evaluated in a hindlimb suspended rat model. Bone mineral density (BMD) was measured by dual-energy X-ray absorptiometry, and the micro-architecture observed by MicroCT assay with bone biomechanical properties evaluated by a three-point bending test. To elucidate potential mechanisms, the osteogenic differentiation effect of baicalin as the most abundant ingredient in RSE was investigated in rat bone marrow derived mesenchymal stem cells (rBMSC). After drug administration for 42 days, tibia-BMD was significantly increased to 0.176 ± 0.007 and 0.183 ± 0.011 g/cm2 and f-BMD was enhanced to 0.200 ± 0.017 and 0.207 ± 0.021 g/cm2 for RSE and ALE treatment, respectively, whereas tibia-BMD and femur-BMD of the HLS group were 0.157 ± 0.009 and 0.176 ± 0.008 g/cm2. Deterioration of bone trabecula microstructure was improved by RSE and ALE with increased morphological parameters such as bone volume fraction, trabecular thickness, and trabecular number, as well as connectivity density compared to the HLS group (p < 0.01). A three-point bending test suggested that bone mechanical strength was also enhanced by RSE and ALE treatments with increased maximum stress, young’s modulus, maximum load, and stiffness compared to those of the HLS group (p < 0.05). Besides, serum TRACP levels were significantly suppressed by RSE and ALE treatments. Furthermore, in vitro studies demonstrated that baicalin significantly increased ALP activities and the formation of mineralized nodules in rBMSC. Conclusively, supplementation of RSE could significantly prevent weightlessness induced osteoporosis, which might attribute to the osteogenic differentiation enhancement effect of baicalin. View Full-Text
Keywords: Radix Scutellariae; baicalin; disuse-induced osteoporosis; osteogenic differentiation; bone marrow derived mesenchymal stem cells Radix Scutellariae; baicalin; disuse-induced osteoporosis; osteogenic differentiation; bone marrow derived mesenchymal stem cells
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Zhang, G.; Li, C.; Niu, Y.; Yu, Q.; Chen, Y.; Liu, E. Osteoprotective Effect of Radix Scutellariae in Female Hindlimb-Suspended Sprague-Dawley Rats and the Osteogenic Differentiation Effect of Its Major Constituent. Molecules 2017, 22, 1044.

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