Next Article in Journal
Protective Effect of Caffeic Acid Derivatives on tert-Butyl Hydroperoxide-Induced Oxidative Hepato-Toxicity and Mitochondrial Dysfunction in HepG2 Cells
Next Article in Special Issue
Synthesis of Aminoglycoside-2′-O-Methyl Oligoribonucleotide Fusions
Previous Article in Journal
Chemical Composition, Antioxidant and α-Glucosidase-Inhibiting Activities of the Aqueous and Hydroethanolic Extracts of Vaccinium myrtillus Leaves
Previous Article in Special Issue
Construction and Biological Evaluation of a Novel Integrin ανβ3-Specific Carrier for Targeted siRNA Delivery In Vitro
Article Menu
Issue 5 (May) cover image

Export Article

Open AccessArticle
Molecules 2017, 22(5), 706; doi:10.3390/molecules22050706

Generation and Applications of a DNA Aptamer against Gremlin-1

1
Department of Rheumatology, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, China
2
Research Center of Reproductive Medicine, Medical School of Xi’an Jiaotong University, Xi’an 710061, China
3
Department of Infectious Diseases, the Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Harri Lönnberg
Received: 16 March 2017 / Revised: 15 April 2017 / Accepted: 22 April 2017 / Published: 28 April 2017
(This article belongs to the Special Issue Synthesis and Applications of Oligonucleotide Conjugates)
View Full-Text   |   Download PDF [2444 KB, uploaded 28 April 2017]   |  

Abstract

Gremlin-1, a highly conserved glycosylated and phosphorylated secretory protein, plays important roles in diverse biological processes including early embryonic development, fibrosis, tumorigenesis, and renal pathophysiology. Aptamers, which are RNA or DNA single-stranded oligonucleotides capable of binding specifically to different targets ranging from small organics to whole cells, have potential applications in targeted imaging, diagnosis and therapy. In this study, we obtained a DNA aptamer against Gremlin-1 (G-ap49) using in vitro Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Binding assay and dot-blot showed that G-ap49 had high affinity for Gremlin-1. Further experiments indicated that G-ap49 was quite stable in a cell culture system and could be used in South-Western blot analysis, enzyme-linked aptamer sorbent assay (ELASA), and aptamer-based cytochemistry and histochemistry staining to detect Gremlin-1. Moreover, our study demonstrated that G-ap49 is capable of revealing the subcellular localization of Gremlin-1. These data indicate that G-ap49 can be used as an alternative to antibodies in detecting Gremlin-1. View Full-Text
Keywords: aptamer; Gremlin-1; South-Western blot analysis; enzyme-linked aptamer sorbent assay; immunohisto-cytochemical staining aptamer; Gremlin-1; South-Western blot analysis; enzyme-linked aptamer sorbent assay; immunohisto-cytochemical staining
Figures

Figure 1a

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Li, Q.; Huo, Y.; Guo, Y.; Zheng, X.; Sun, W.; Hao, Z. Generation and Applications of a DNA Aptamer against Gremlin-1. Molecules 2017, 22, 706.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top