Response of Ustilago maydis against the Stress Caused by Three Polycationic Chitin Derivatives
AbstractChitosan is a stressing molecule that affects the cells walls and plasma membrane of fungi. For chitosan derivatives, the action mode is not clear. In this work, we used the yeast Ustilago maydis to study the effects of these molecules on the plasma membrane, focusing on physiologic and stress responses to chitosan (CH), oligochitosan (OCH), and glycol-chitosan (GCH). Yeasts were cultured with each of these molecules at 1 mg·mL−1 in minimal medium. To compare plasma membrane damage, cells were cultivated in isosmolar medium. Membrane potential (Δψ) as well as oxidative stress were measured. Changes in the total plasma membrane phospholipid and protein profiles were analyzed using standard methods, and fluorescence-stained mitochondria were observed. High osmolarity did not protect against CH inhibition and neither affected membrane potential. The OCH did produce higher oxidative stress. The effects of these molecules were evidenced by modifications in the plasma membrane protein profile. Also, mitochondrial damage was evident for CH and OCH, while GCH resulted in thicker cells with fewer mitochondria and higher glycogen accumulation. View Full-Text
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Olicón-Hernández, D.R.; Uribe-Alvarez, C.; Uribe-Carvajal, S.; Pardo, J.P.; Guerra-Sánchez, G. Response of Ustilago maydis against the Stress Caused by Three Polycationic Chitin Derivatives. Molecules 2017, 22, 1745.
Olicón-Hernández DR, Uribe-Alvarez C, Uribe-Carvajal S, Pardo JP, Guerra-Sánchez G. Response of Ustilago maydis against the Stress Caused by Three Polycationic Chitin Derivatives. Molecules. 2017; 22(12):1745.Chicago/Turabian Style
Olicón-Hernández, Dario R.; Uribe-Alvarez, Cristina; Uribe-Carvajal, Salvador; Pardo, Juan P.; Guerra-Sánchez, Guadalupe. 2017. "Response of Ustilago maydis against the Stress Caused by Three Polycationic Chitin Derivatives." Molecules 22, no. 12: 1745.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.