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Molecules 2017, 22(12), 2157; doi:10.3390/molecules22122157

Improved Performance of Magnetic Cross-Linked Lipase Aggregates by Interfacial Activation: A Robust and Magnetically Recyclable Biocatalyst for Transesterification of Jatropha Oil

1
School of Chemistry and Chemical Engineering, National Demonstration Center for Experimental Chemistry Education, Ningxia University, Yinchuan 750021, China
2
Key Laboratory of Green Chemistry Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, China
*
Authors to whom correspondence should be addressed.
Received: 18 November 2017 / Revised: 28 November 2017 / Accepted: 2 December 2017 / Published: 7 December 2017
(This article belongs to the Special Issue Lipases and Lipases Modification)
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Abstract

Lipases are the most widely employed enzymes in commercial industries. The catalytic mechanism of most lipases involves a step called “interfacial activation”. As interfacial activation can lead to a significant increase in catalytic activity, it is of profound importance in developing lipase immobilization methods. To obtain a potential biocatalyst for industrial biodiesel production, an effective strategy for enhancement of catalytic activity and stability of immobilized lipase was developed. This was performed through the combination of interfacial activation with hybrid magnetic cross-linked lipase aggregates. This biocatalyst was investigated for the immobilization of lipase from Rhizomucor miehei (RML). Under the optimal conditions, the activity recovery of the surfactant-activated magnetic RML cross-linked enzyme aggregates (CLEAs) was as high as 2058%, with a 20-fold improvement over the free RML. Moreover, the immobilized RML showed excellent catalytic performance for the biodiesel reaction at a yield of 93%, and more importantly, could be easily separated from the reaction mixture by simple magnetic decantation, and retained more than 84% of its initial activities after five instances of reuse. This study provides a new and versatile approach for designing and fabricating immobilized lipase with high activation and stability. View Full-Text
Keywords: lipase; interfacial activation; surfactant; magnetic nanoparticles; cross-linked enzyme aggregates; enzyme immobilization; biodiesel lipase; interfacial activation; surfactant; magnetic nanoparticles; cross-linked enzyme aggregates; enzyme immobilization; biodiesel
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Zhang, W.; Yang, H.; Liu, W.; Wang, N.; Yu, X. Improved Performance of Magnetic Cross-Linked Lipase Aggregates by Interfacial Activation: A Robust and Magnetically Recyclable Biocatalyst for Transesterification of Jatropha Oil. Molecules 2017, 22, 2157.

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