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Molecules 2017, 22(1), 179; doi:10.3390/molecules22010179

Co-Immobilization of Enzymes and Magnetic Nanoparticles by Metal-Nucleotide Hydrogelnanofibers for Improving Stability and Recycling

1
Department of Environment Protection and Detection, Beijing Industrial Technician College, Beijing 100023, China
2
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China
3
Beijing Centre for Physical and Chemical Analysis, Beijing 100089, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Roberto Fernandez-Lafuente
Received: 13 November 2016 / Revised: 13 January 2017 / Accepted: 17 January 2017 / Published: 23 January 2017
(This article belongs to the Special Issue Enzyme Immobilization 2016)
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Abstract

In this paper we report a facile method for preparing co-immobilized enzyme and magnetic nanoparticles (MNPs) using metal coordinated hydrogel nanofibers. Candida rugosa lipase (CRL) was selected as guest protein. For good aqueous dispersity, low price and other unique properties, citric acid-modified magnetic iron oxide nanoparticles (CA-Fe3O4 NPs) have been widely used for immobilizing enzymes. As a result, the relative activity of CA-Fe3O4@Zn/AMP nanofiber-immobilized CRL increased by 8-fold at pH 10.0 and nearly 1-fold in a 50 °C water bath after 30 min, compared to free CRL. Moreover, the immobilized CRL had excellent long-term storage stability (nearly 80% releative activity after storage for 13 days). This work indicated that metal-nucleotide nanofibers could efficiently co-immobilize enzymes and MNPs simultaneously, and improve the stability of biocatalysts. View Full-Text
Keywords: metal; nucleotide; magnetic iron oxide nanoparticles; Candida rugose lipase (CRL); immobilized enzyme metal; nucleotide; magnetic iron oxide nanoparticles; Candida rugose lipase (CRL); immobilized enzyme
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Li, C.; Jiang, S.; Zhao, X.; Liang, H. Co-Immobilization of Enzymes and Magnetic Nanoparticles by Metal-Nucleotide Hydrogelnanofibers for Improving Stability and Recycling. Molecules 2017, 22, 179.

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