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Molecules 2017, 22(1), 187; doi:10.3390/molecules22010187

Isolation and Characterization of a Phaseolus vulgaris Trypsin Inhibitor with Antiproliferative Activity on Leukemia and Lymphoma Cells

1
State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China
2
Engineering Research Center of System-Nutrition, Beijing Research Institute for Nutritional Resources, Beijing 100069, China
3
Institute of Plant Nutrition, Agricultural Resources and Environmental Science, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
4
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
*
Author to whom correspondence should be addressed.
Academic Editors: Zhaohui Sunny Zhou and Derek J. McPhee
Received: 6 December 2016 / Revised: 5 January 2017 / Accepted: 17 January 2017 / Published: 23 January 2017
View Full-Text   |   Download PDF [650 KB, uploaded 23 January 2017]   |  

Abstract

A 17.5-kDa trypsin inhibitor was purified from Phaseolus vulgaris cv. “gold bean” with an isolation protocol including ion exchange chromatography on DEAE-cellulose (Diethylaminoethyl-cellulose), affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-sepharose (Sulfopropyl-sepharose), and gel filtration by FPLC (Fast protein liquid chromatography) on Superdex 75. It dose-dependently inhibited trypsin with an IC50 value of 0.4 μM, and this activity was reduced in the presence of dithiothreitol in a dose- and time-dependent manner, signifying the importance of the disulfide linkage to the activity. It inhibited [methyl-3H] thymidine incorporation by leukemia L1210 cells and lymphoma MBL2 cells with an IC50 value of 2.3 μM and 2.5 μM, respectively. The inhibitor had no effect on fungal growth and the activities of various viral enzymes when tested up to 100 μM. View Full-Text
Keywords: leguminous seeds; leukemia L1210; lymphoma MBL2; purification leguminous seeds; leukemia L1210; lymphoma MBL2; purification
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Li, M.; Liu, Q.; Cui, Y.; Li, D.; Wang, H.; Ng, T.B. Isolation and Characterization of a Phaseolus vulgaris Trypsin Inhibitor with Antiproliferative Activity on Leukemia and Lymphoma Cells. Molecules 2017, 22, 187.

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