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Molecules 2016, 21(6), 779; doi:10.3390/molecules21060779

Chemical Structure and Immunomodulating Activities of an α-Glucan Purified from Lobelia chinensis Lour

1
Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
2
School of Chinese Medicine, Hong Kong Baptist University, Hong Kong 999077, China
3
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao 999078, China
4
Department of Chemistry, Hong Kong Baptist University, Hong Kong 999077, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 3 May 2016 / Revised: 3 June 2016 / Accepted: 7 June 2016 / Published: 15 June 2016
(This article belongs to the Special Issue Natural Polysaccharides)
View Full-Text   |   Download PDF [3349 KB, uploaded 15 June 2016]   |  

Abstract

A neutral α-glucan, named BP1, with a molecular mass of approximately 9.45 kDa, was isolated from Lobelia chinensis by hot-water extraction, a Q-Sepharose Fast Flow column and Superdex-75 column chromatography. Its chemical structure was characterized by monosaccharide analysis, methylation analysis and analysis of its FT-IR, high performance gel permeation chromatography (HPGPC) and 1D/2D-NMR spectra data. The backbone of BP1 consists of →6α-d-Glcp16,3α-d-Glcp1→(6α-d-Glcp1)x-6,3α-d-Glcp1-(6α-d-Glcp1)y→. The side chains were terminal α-d-Glcp1→ and α-d-Glcp1→ (6α-d-Glcp1)z→4α-d-Glcp13α-d-Glcp14α-d-Glcp1→ (x + y + z = 5), which are attached to the backbone at O-3 of 3,6α-d-Glcp1. The results of the effect of BP1 on mouse macrophage cell line RAW 264.7 indicate that BP1 enhances the cell proliferation, phagocytosis, nitric oxide production and cytokine secretion in a dose-dependent manner. Because the inhibitor of Toll-like receptor 4 blocks the BP1-induced secretion of TNF-α and IL-6, we hypothesize that α-glucan BP1 activates TLR4, which mediates the above-mentioned immunomodulating effects. View Full-Text
Keywords: Lobelia chinensis; NMR; RAW 264.7; immunomodulating; TLR4 Lobelia chinensis; NMR; RAW 264.7; immunomodulating; TLR4
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Li, X.-J.; Bao, W.-R.; Leung, C.-H.; Ma, D.-L.; Zhang, G.; Lu, A.-P.; Wang, S.-C.; Han, Q.-B. Chemical Structure and Immunomodulating Activities of an α-Glucan Purified from Lobelia chinensis Lour. Molecules 2016, 21, 779.

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