Chemical Structure and Immunomodulating Activities of an α-Glucan Purified from Lobelia chinensis Lour
AbstractA neutral α-glucan, named BP1, with a molecular mass of approximately 9.45 kDa, was isolated from Lobelia chinensis by hot-water extraction, a Q-Sepharose Fast Flow column and Superdex-75 column chromatography. Its chemical structure was characterized by monosaccharide analysis, methylation analysis and analysis of its FT-IR, high performance gel permeation chromatography (HPGPC) and 1D/2D-NMR spectra data. The backbone of BP1 consists of →6α-d-Glcp1→6,3α-d-Glcp1→(6α-d-Glcp1)x-6,3α-d-Glcp1-(6α-d-Glcp1)y→. The side chains were terminal α-d-Glcp1→ and α-d-Glcp1→ (6α-d-Glcp1)z→4α-d-Glcp1→3α-d-Glcp1→4α-d-Glcp1→ (x + y + z = 5), which are attached to the backbone at O-3 of 3,6α-d-Glcp1. The results of the effect of BP1 on mouse macrophage cell line RAW 264.7 indicate that BP1 enhances the cell proliferation, phagocytosis, nitric oxide production and cytokine secretion in a dose-dependent manner. Because the inhibitor of Toll-like receptor 4 blocks the BP1-induced secretion of TNF-α and IL-6, we hypothesize that α-glucan BP1 activates TLR4, which mediates the above-mentioned immunomodulating effects. View Full-Text
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Li, X.-J.; Bao, W.-R.; Leung, C.-H.; Ma, D.-L.; Zhang, G.; Lu, A.-P.; Wang, S.-C.; Han, Q.-B. Chemical Structure and Immunomodulating Activities of an α-Glucan Purified from Lobelia chinensis Lour. Molecules 2016, 21, 779.
Li X-J, Bao W-R, Leung C-H, Ma D-L, Zhang G, Lu A-P, Wang S-C, Han Q-B. Chemical Structure and Immunomodulating Activities of an α-Glucan Purified from Lobelia chinensis Lour. Molecules. 2016; 21(6):779.Chicago/Turabian Style
Li, Xiao-Jun; Bao, Wan-Rong; Leung, Chung-Hang; Ma, Dik-Lung; Zhang, Ge; Lu, Ai-Ping; Wang, Shun-Chun; Han, Quan-Bin. 2016. "Chemical Structure and Immunomodulating Activities of an α-Glucan Purified from Lobelia chinensis Lour." Molecules 21, no. 6: 779.
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