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Molecules 2016, 21(2), 32; doi:10.3390/molecules21020032

Functional Characterization of a Dihydroflavanol 4-Reductase from the Fiber of Upland Cotton (Gossypium hirsutum)

1
The State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
2
Hunan Provincial Key Laboratory of Plant Resources Conservation and Utilization, College of Biology and Environmental Sciences, Jishou University, No. 120 Ren Min South Road, Jishou 416000, Hunan, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editors: Luis Cisneros-Zevallos and Daniel Jacobo-Velazquez
Received: 30 September 2015 / Revised: 10 December 2015 / Accepted: 21 December 2015 / Published: 26 January 2016
(This article belongs to the Special Issue Recent Advances in Plant Phenolics)
View Full-Text   |   Download PDF [5254 KB, uploaded 26 January 2016]   |  

Abstract

Dihydroflavanol 4-reductase (DFR) is a key later enzyme involved in two polyphenols’ (anthocyanins and proanthocyanidins (PAs)) biosynthesis, however it is not characterized in cotton yet. In present reports, a DFR cDNA homolog (designated as GhDFR1) was cloned from developing fibers of upland cotton. Silencing GhDFR1 in cotton by virus-induced gene silencing led to significant decrease in accumulation of anthocyanins and PAs. More interestingly, based on LC-MS analysis, two PA monomers, (–)-epicatachin and (–)-epigallocatachin, remarkably decreased in content in fibers of GhDFR1-silenced plants, but two new monomers, (–)-catachin and (–)-gallocatachin were present compared to the control plants infected with empty vector. The ectopic expression of GhDFR1 in an Arabidopsis TT3 mutant allowed for reconstruction of PAs biosynthesis pathway and led to accumulation of PAs in seed coat. Taken together, these data demonstrate that GhDFR1 contributes to the biosynthesis of anthocyanins and PAs in cotton. View Full-Text
Keywords: upland cotton (Gossypium hirsutum); dihydroflavanol-4-reductase (DFR); anthocyanins; proanthocyanidins (PAs); flavan-3-ols upland cotton (Gossypium hirsutum); dihydroflavanol-4-reductase (DFR); anthocyanins; proanthocyanidins (PAs); flavan-3-ols
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Wang, L.; Zhu, Y.; Wang, P.; Fan, Q.; Wu, Y.; Peng, Q.-Z.; Xia, G.-X.; Wu, J.-H. Functional Characterization of a Dihydroflavanol 4-Reductase from the Fiber of Upland Cotton (Gossypium hirsutum). Molecules 2016, 21, 32.

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