Multi-Functional Nanogels for Tumor Targeting and Redox-Sensitive Drug and siRNA Delivery
Abstract(1) Background: A new family of nanosystems able to discern between normal and tumor cells and to release a therapeutic agent in controlled way were synthetized by e-beam irradiation. This technique permits to obtain biocompatible, sterile, carboxyl-functionalized polyvinylpyrrolidone (PVP-co-acrylic acid) nanogels (NGs); (2) Methods: Here, we performed a targeting strategy based on the recognition of over-expressed proteins on tumor cells, like the folate receptor. The selective targeting was demonstrated by co-culture studies and flow cytometry analysis, using folate conjugated NGs. Moreover, nanoparticles were conjugated to a chemotherapeutic drug or to a pro-apoptotic siRNA through a glutathione sensitive spacer, in order to obtain a controlled release mechanism, specific for cancer cells. The drug efficiency was tested on tumor and healthy cells by flow cytometric analysis, confocal and epifluorescence microscopy and cytotoxicity assay; the siRNA effect was investigated by RNAi experiment; (3) Results: The data obtained showed that the use of NGs permits a faster cargo release in cancer cells, in response to high cytosolic glutathione level, also improving their efficacy; (4) Conclusion: The possibility of releasing biological molecules in a controlled way and to recognize a specific tumor target allows overcoming the typical limits of the classic cancer therapy. View Full-Text
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Adamo, G.; Grimaldi, N.; Campora, S.; Bulone, D.; Bondì, M.L.; Al-Sheikhly, M.; Sabatino, M.A.; Dispenza, C.; Ghersi, G. Multi-Functional Nanogels for Tumor Targeting and Redox-Sensitive Drug and siRNA Delivery. Molecules 2016, 21, 1594.
Adamo G, Grimaldi N, Campora S, Bulone D, Bondì ML, Al-Sheikhly M, Sabatino MA, Dispenza C, Ghersi G. Multi-Functional Nanogels for Tumor Targeting and Redox-Sensitive Drug and siRNA Delivery. Molecules. 2016; 21(11):1594.Chicago/Turabian Style
Adamo, Giorgia; Grimaldi, Natascia; Campora, Simona; Bulone, Donatella; Bondì, Maria L.; Al-Sheikhly, Mohamad; Sabatino, Maria A.; Dispenza, Clelia; Ghersi, Giulio. 2016. "Multi-Functional Nanogels for Tumor Targeting and Redox-Sensitive Drug and siRNA Delivery." Molecules 21, no. 11: 1594.
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