Enzyme Engineering for In Situ Immobilization
AbstractEnzymes are used as biocatalysts in a vast range of industrial applications. Immobilization of enzymes to solid supports or their self-assembly into insoluble particles enhances their applicability by strongly improving properties such as stability in changing environments, re-usability and applicability in continuous biocatalytic processes. The possibility of co-immobilizing various functionally related enzymes involved in multistep synthesis, conversion or degradation reactions enables the design of multifunctional biocatalyst with enhanced performance compared to their soluble counterparts. This review provides a brief overview of up-to-date in vitro immobilization strategies while focusing on recent advances in enzyme engineering towards in situ self-assembly into insoluble particles. In situ self-assembly approaches include the bioengineering of bacteria to abundantly form enzymatically active inclusion bodies such as enzyme inclusions or enzyme-coated polyhydroxyalkanoate granules. These one-step production strategies for immobilized enzymes avoid prefabrication of the carrier as well as chemical cross-linking or attachment to a support material while the controlled oriented display strongly enhances the fraction of accessible catalytic sites and hence functional enzymes. View Full-Text
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Rehm, F.B.H.; Chen, S.; Rehm, B.H.A. Enzyme Engineering for In Situ Immobilization. Molecules 2016, 21, 1370.
Rehm FBH, Chen S, Rehm BHA. Enzyme Engineering for In Situ Immobilization. Molecules. 2016; 21(10):1370.Chicago/Turabian Style
Rehm, Fabian B.H.; Chen, Shuxiong; Rehm, Bernd H.A. 2016. "Enzyme Engineering for In Situ Immobilization." Molecules 21, no. 10: 1370.
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