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Molecules 2016, 21(10), 1300; doi:10.3390/molecules21101300

Flavonoid Interaction with a Chitinase from Grape Berry Skin: Protein Identification and Modulation of the Enzymatic Activity

1
Department of Agricultural, Food, Animal and Environmental Sciences, University of Udine, Via delle Scienze 91, 33100 Udine, Italy
2
Blood Transfusion Centre of Slovenia, Department of R&D, Šlajmerjeva 6, 1000 Ljubljana, Slovenia
3
Department of Life Sciences, University of Trieste, via L. Giorgieri 1, 34127 Trieste, Italy
4
Proteomics and Mass Spectrometry Laboratory, ISPAAM, CNR, via Argine, 1085-80147 Napoli, Italy
*
Author to whom correspondence should be addressed.
Academic Editors: Celestino Santos-Buelga and Arturo San Feliciano
Received: 1 September 2016 / Revised: 20 September 2016 / Accepted: 22 September 2016 / Published: 28 September 2016
(This article belongs to the Special Issue Flavonoids: From Structure to Health Issues)
View Full-Text   |   Download PDF [2725 KB, uploaded 29 September 2016]   |  

Abstract

In the present study, an antibody raised against a peptide sequence of rat bilitranslocase (anti-peptide Ab) was tested on microsomal proteins obtained from red grape berry skin. Previously, this antibody had demonstrated to recognize plant membrane proteins associated with flavonoid binding and transport. Immuno-proteomic assays identified a number of proteins reacting with this particular antibody, suggesting that the flavonoid binding and interaction may be extended not only to carriers of these molecules, but also to enzymes with very different functions. One of these proteins is a pathogenesis-related (PR) class IV chitinase, whose in vitro chitinolytic activity was modulated by two of the most representative flavonoids of grape, quercetin and catechin, as assessed by both spectrophotometric and fluorimetric assays in grape microsomes and commercial enzyme preparations. The effect of these flavonoids on the catalysis and its kinetic parameters was also evaluated, evidencing that they determine a hormetic dose-dependent response. These results highlight the importance of flavonoids not only as antioxidants or antimicrobial effectors, but also as modulators of plant growth and stress response. Implications of the present suggestion are here discussed in the light of environment and pesticide-reduction concerns. View Full-Text
Keywords: catechin; chitinase; pathogenesis-related proteins; quercetin; Vitis vinifera catechin; chitinase; pathogenesis-related proteins; quercetin; Vitis vinifera
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Filippi, A.; Petrussa, E.; Rajcevic, U.; Čurin Šerbec, V.; Passamonti, S.; Renzone, G.; Scaloni, A.; Zancani, M.; Vianello, A.; Braidot, E. Flavonoid Interaction with a Chitinase from Grape Berry Skin: Protein Identification and Modulation of the Enzymatic Activity. Molecules 2016, 21, 1300.

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