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Molecules 2016, 21(10), 1409; doi:10.3390/molecules21101409

A Fast and Robust UHPLC-MRM-MS Method to Characterize and Quantify Grape Skin Tannins after Chemical Depolymerization

1
Plate-Forme D’analyse des Polyphénols, UMR1083 Sciences Pour l’Œnologie, Institut National de la Recherche Agronomique, Montpellier 34060, France
2
Equipe Biosynthèse et Composition en Polyphénols et Polysaccharides, UMR1083 Sciences Pour l’Œnologie, Institut National de la Recherche Agronomique, Montpellier 34060, France
3
Institut Français de la Vigne et du Vin, Pôle National Matériel Végétal, UMT Génovigne, Montpellier 34060, France
*
Author to whom correspondence should be addressed.
Academic Editors: Arturo San Feliciano and Celestino Santos-Buelga
Received: 30 September 2016 / Revised: 18 October 2016 / Accepted: 19 October 2016 / Published: 21 October 2016
(This article belongs to the Special Issue Flavonoids: From Structure to Health Issues)
View Full-Text   |   Download PDF [2288 KB, uploaded 21 October 2016]   |  

Abstract

A rapid, sensitive, and selective analysis method using ultra high performance liquid chromatography coupled with triple-quadrupole mass spectrometry (UHPLC-QqQ-MS) has been developed for the characterization and quantification of grape skin flavan-3-ols after acid-catalysed depolymerization in the presence of phloroglucinol (phloroglucinolysis). The compound detection being based on specific MS transitions in Multiple Reaction Monitoring (MRM) mode, this fast gradient robust method allows analysis of constitutive units of grape skin proanthocyanidins, including some present in trace amounts, in a single injection, with a throughput of 6 samples per hour. This method was applied to a set of 214 grape skin samples from 107 different red and white grape cultivars grown under two conditions in the vineyard, irrigated or non-irrigated. The results of triplicate analyses confirmed the robustness of the method, which was thus proven to be suitable for high-throughput and large-scale metabolomics studies. Moreover, these preliminary results suggest that analysis of tannin composition is relevant to investigate the genetic bases of grape response to drought. View Full-Text
Keywords: grapes; tannins; phloroglucinolysis; quantitative UHPLC-QqQ-MS; method validation; metabolomics grapes; tannins; phloroglucinolysis; quantitative UHPLC-QqQ-MS; method validation; metabolomics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Pinasseau, L.; Verbaere, A.; Roques, M.; Meudec, E.; Vallverdú-Queralt, A.; Terrier, N.; Boulet, J.-C.; Cheynier, V.; Sommerer, N. A Fast and Robust UHPLC-MRM-MS Method to Characterize and Quantify Grape Skin Tannins after Chemical Depolymerization. Molecules 2016, 21, 1409.

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