Molecules 2014, 19(3), 3038-3054; doi:10.3390/molecules19033038
Article

Assessment of Circulating microRNAs in Plasma of Lung Cancer Patients

Received: 17 February 2014; in revised form: 28 February 2014 / Accepted: 4 March 2014 / Published: 10 March 2014
(This article belongs to the Special Issue miRNAs as Probes to Monitor Cancer and Neurodegenerative Disorders)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Lung cancer is the most common cause of cancer deaths worldwide and numerous ongoing research efforts are directed to identify new strategies for its early detection. The development of non-invasive blood-based biomarkers for cancer detection in its preclinical phases is crucial to improve the outcome of this deadly disease. MicroRNAs (miRNAs) are a new promising class of circulating biomarkers for cancer detection and prognosis definition, but lack of consensus on data normalization methods for circulating miRNAs and the critical issue of haemolysis, has affected the identification of circulating miRNAs with diagnostic potential. We describe here an interesting approach for profiling circulating miRNAs in plasma samples based on the evaluation of reciprocal miRNA levels measured by quantitative Real-Time PCR. By monitoring changes of plasma miRNA-ratios, it is possible to assess the deregulation of tumor-related miRNAs and identify signatures with diagnostic and prognostic value. In addition, to avoid bias due to the release of miRNAs from blood cells, a miRNA-ratios signature distinguishing haemolyzed samples was identified. The method described was validated in plasma samples of lung cancer patients, but given its reproducibility and reliability, could be potentially applied for the identification of diagnostic circulating miRNAs in other diseases.
Keywords: miRNAs; biomarkers; early diagnosis; lung cancer; real-time PCR; haemolysis
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MDPI and ACS Style

Fortunato, O.; Boeri, M.; Verri, C.; Conte, D.; Mensah, M.; Suatoni, P.; Pastorino, U.; Sozzi, G. Assessment of Circulating microRNAs in Plasma of Lung Cancer Patients. Molecules 2014, 19, 3038-3054.

AMA Style

Fortunato O, Boeri M, Verri C, Conte D, Mensah M, Suatoni P, Pastorino U, Sozzi G. Assessment of Circulating microRNAs in Plasma of Lung Cancer Patients. Molecules. 2014; 19(3):3038-3054.

Chicago/Turabian Style

Fortunato, Orazio; Boeri, Mattia; Verri, Carla; Conte, Davide; Mensah, Mavis; Suatoni, Paola; Pastorino, Ugo; Sozzi, Gabriella. 2014. "Assessment of Circulating microRNAs in Plasma of Lung Cancer Patients." Molecules 19, no. 3: 3038-3054.

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