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Molecules 2014, 19(3), 3025-3037; doi:10.3390/molecules19033025
Article

Microwave-Assisted Extraction of Herbacetin Diglucoside from Flax (Linum usitatissimum L.) Seed Cakes and Its Quantification using an RP-HPLC-UV System

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1 Laboratoire de Phytotechnologie EA 3900 BIOPI, Faculté de Pharmacie, Université de Picardie Jules Verne, 1 rue des Louvels, 80037 Amiens, France 2 Laboratoire de Biologie des Ligneux et des Grandes Cultures UPRES EA 1207, Equipe Lignanes des Linacées, Université d'Orléans — Antenne Scientifique Universitaire de Chartres, 21 rue de Loigny la Bataille, 28000 Chartres, France 3 Génie Enzymatique et Cellulaire, FRE 3580 CNRS, Université de Picardie Jules Verne, 33 rue Saint-Leu, 80039 Amiens, France 4 Laboratoire des Glucides FRE 3517 CNRS, Faculté des Sciences, Université de Picardie Jules Verne, 33 rue Saint-Leu, 80039 Amiens, France 5 Ecole SITI (Département CASER), Conservatoire National des Arts et Métiers, 292 rue Saint Martin, 75141 Paris Cedex 03, France 6 Service de Transformations Chimiques et Pharmaceutiques, ERL CNRS 3193, Conservatoire National des Arts et Métiers, 2 rue Conté, 75003 Paris, France These two authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 9 January 2014 / Revised: 26 February 2014 / Accepted: 28 February 2014 / Published: 10 March 2014
(This article belongs to the Section Metabolites)
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Abstract

Flax (Linum usitatissimum L.) seeds are widely used for oil extraction and the cold-pressed flaxseed (or linseed) cakes obtained during this process constitute a valuable by-product. The flavonol herbacetin diglucoside (HDG) has been previously reported as a constituent of the flaxseed lignan macromolecule linked through ester bonds to the linker molecule hydroxymethylglutaric acid. In this context, the development and validation of a new approach using microwave-assisted extraction (MAE) of HDG from flaxseed cakes followed by quantification with a reverse-phase HPLC system with UV detection was purposed. The experimental parameters affecting the HDG extraction yield, such as microwave power, extraction time and sodium hydroxide concentration, from the lignan macromolecule were optimized. A maximum HDG concentration of 5.76 mg/g DW in flaxseed cakes was measured following an irradiation time of 6 min, for a microwave power of 150 W using a direct extraction in 0.1 M NaOH in 70% (v/v) aqueous methanol. The optimized method was proven to be rapid and reliable in terms of precision, repeatability, stability and accuracy for the extraction of HDG. Comparison with a conventional extraction method demonstrated that MAE is more effective and less time-consuming.
Keywords: flavonol; flaxseed; herbacetin diglucoside; Linum usitatissimum; microwave-assisted extraction flavonol; flaxseed; herbacetin diglucoside; Linum usitatissimum; microwave-assisted extraction
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fliniaux, O.; Corbin, C.; Ramsay, A.; Renouard, S.; Beejmohun, V.; Doussot, J.; Falguières, A.; Ferroud, C.; Lamblin, F.; Lainé, E.; Roscher, A.; Grand, E.; Mesnard, F.; Hano, C. Microwave-Assisted Extraction of Herbacetin Diglucoside from Flax (Linum usitatissimum L.) Seed Cakes and Its Quantification using an RP-HPLC-UV System. Molecules 2014, 19, 3025-3037.

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