Molecules 2013, 18(6), 7023-7034; doi:10.3390/molecules18067023
Article

A Soft Coral Natural Product, 11-Episinulariolide Acetate, Inhibits Gene Expression of Cyclooxygenase-2 and Interleukin-8 through Attenuation of Calcium Signaling

1,2, 1, 1, 1, 3,4,5, 1, 3,4,5, 5,6, 6,7,8,* email and 1,5,9,10,* email
Received: 22 April 2013; in revised form: 30 May 2013 / Accepted: 13 June 2013 / Published: 17 June 2013
(This article belongs to the Special Issue Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Epidermal growth factor receptor (EGFR) is overexpressed in many types of cancer cells. EGFR-mediated signaling involves inflammatory gene expression including cyclooxygenase (COX)-2 and interleukin (IL)-8, and is associated with cancer pathogenesis. In a search of phytochemicals with anti-inflammatory activity, the COX-2 and IL-8 inhibitory activities of some marine compounds were examined. After screening these compounds 11-episinulariolide acetate (1) from soft coral exhibited the most potent activity. Reverse-transcription PCR; western blotting; ELISA and luciferase assays were used to test the effect of compound 1 on EGF-stimulated expressions of COX-2 and IL-8 in A431 human epidermoid carcinoma cells. After exposure to 10 μM of compound 1, expression levels of COX-2 and IL-8 were reduced. In addition; intracellular Ca2+ increase and Ca2+-dependent transcription factor activation were blocked by compound 1. Thus, compound 1 can potentially serve as a lead compound for targeting Ca2+ signaling-dependent inflammatory diseases.
Keywords: marine compound; EGF; COX-2; IL-8; calcium
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MDPI and ACS Style

Hsu, W.-L.; Chiu, S.-J.; Tsai, Y.-T.; Chang, C.-M.; Wang, J.-Y.; Wang, E.T.; Hou, M.-F.; Huang, C.-Y.; Sheu, J.-H.; Chang, W.-C. A Soft Coral Natural Product, 11-Episinulariolide Acetate, Inhibits Gene Expression of Cyclooxygenase-2 and Interleukin-8 through Attenuation of Calcium Signaling. Molecules 2013, 18, 7023-7034.

AMA Style

Hsu W-L, Chiu S-J, Tsai Y-T, Chang C-M, Wang J-Y, Wang ET, Hou M-F, Huang C-Y, Sheu J-H, Chang W-C. A Soft Coral Natural Product, 11-Episinulariolide Acetate, Inhibits Gene Expression of Cyclooxygenase-2 and Interleukin-8 through Attenuation of Calcium Signaling. Molecules. 2013; 18(6):7023-7034.

Chicago/Turabian Style

Hsu, Wen-Li; Chiu, Siou-Jin; Tsai, Yao-Ting; Chang, Che-Mai; Wang, Jaw-Yan; Wang, Eric T.; Hou, Ming-Feng; Huang, Chiung-Yao; Sheu, Jyh-Horng; Chang, Wei-Chiao. 2013. "A Soft Coral Natural Product, 11-Episinulariolide Acetate, Inhibits Gene Expression of Cyclooxygenase-2 and Interleukin-8 through Attenuation of Calcium Signaling." Molecules 18, no. 6: 7023-7034.

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