Molecules 2013, 18(6), 7023-7034; doi:10.3390/molecules18067023
Article

A Soft Coral Natural Product, 11-Episinulariolide Acetate, Inhibits Gene Expression of Cyclooxygenase-2 and Interleukin-8 through Attenuation of Calcium Signaling

1 Department of Clinical Pharmacy, Taipei Medical University, Taipei 110, Taiwan 2 Department of Physiology, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan 3 Division of Gastroeintestinal and General Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, Taiwan 4 Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan 5 Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, Taiwan 6 Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan 7 Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan 8 Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 404, Taiwan 9 Department of Pharmacy, Taipei Medical University-Wanfang Hospital, Taipei 116, Taiwan 10 Master Program for Clinical Pharmacogenomics and Pharmacoproteomics, School of Pharmacy, Taipei Medical University, Taipei 110, Taiwan
* Authors to whom correspondence should be addressed.
Received: 22 April 2013; in revised form: 30 May 2013 / Accepted: 13 June 2013 / Published: 17 June 2013
(This article belongs to the Special Issue Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry)
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Abstract: Epidermal growth factor receptor (EGFR) is overexpressed in many types of cancer cells. EGFR-mediated signaling involves inflammatory gene expression including cyclooxygenase (COX)-2 and interleukin (IL)-8, and is associated with cancer pathogenesis. In a search of phytochemicals with anti-inflammatory activity, the COX-2 and IL-8 inhibitory activities of some marine compounds were examined. After screening these compounds 11-episinulariolide acetate (1) from soft coral exhibited the most potent activity. Reverse-transcription PCR; western blotting; ELISA and luciferase assays were used to test the effect of compound 1 on EGF-stimulated expressions of COX-2 and IL-8 in A431 human epidermoid carcinoma cells. After exposure to 10 μM of compound 1, expression levels of COX-2 and IL-8 were reduced. In addition; intracellular Ca2+ increase and Ca2+-dependent transcription factor activation were blocked by compound 1. Thus, compound 1 can potentially serve as a lead compound for targeting Ca2+ signaling-dependent inflammatory diseases.
Keywords: marine compound; EGF; COX-2; IL-8; calcium

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MDPI and ACS Style

Hsu, W.-L.; Chiu, S.-J.; Tsai, Y.-T.; Chang, C.-M.; Wang, J.-Y.; Wang, E.T.; Hou, M.-F.; Huang, C.-Y.; Sheu, J.-H.; Chang, W.-C. A Soft Coral Natural Product, 11-Episinulariolide Acetate, Inhibits Gene Expression of Cyclooxygenase-2 and Interleukin-8 through Attenuation of Calcium Signaling. Molecules 2013, 18, 7023-7034.

AMA Style

Hsu W-L, Chiu S-J, Tsai Y-T, Chang C-M, Wang J-Y, Wang ET, Hou M-F, Huang C-Y, Sheu J-H, Chang W-C. A Soft Coral Natural Product, 11-Episinulariolide Acetate, Inhibits Gene Expression of Cyclooxygenase-2 and Interleukin-8 through Attenuation of Calcium Signaling. Molecules. 2013; 18(6):7023-7034.

Chicago/Turabian Style

Hsu, Wen-Li; Chiu, Siou-Jin; Tsai, Yao-Ting; Chang, Che-Mai; Wang, Jaw-Yan; Wang, Eric T.; Hou, Ming-Feng; Huang, Chiung-Yao; Sheu, Jyh-Horng; Chang, Wei-Chiao. 2013. "A Soft Coral Natural Product, 11-Episinulariolide Acetate, Inhibits Gene Expression of Cyclooxygenase-2 and Interleukin-8 through Attenuation of Calcium Signaling." Molecules 18, no. 6: 7023-7034.

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