Production of Flavours and Fragrances via Bioreduction of (4R)-(-)-Carvone and (1R)-(-)-Myrtenal by Non-Conventional Yeast Whole-Cells
Abstract
:1. Introduction
2. Results and Discussion
Species and strain | Conversion (mol %) | Products (mol %) | |||||
---|---|---|---|---|---|---|---|
2b | 2a | 3b | 3a | 3d | 3c | ||
C. maltosa DBVPG 6021 | 12.93 ± 3.87 | 9.76 ± 4.49 | 0.55 ± 0.04 | 2.63 ± 0.93 | 0 | 0 | 0 |
Cr. gastricus DBVPG 6057 | 4.71 ± 0.15 | 3.73 ± 0.25 | 0.33 ± 0.03 | 0.66 ± 0.09 | 0 | 0 | 0 |
C. oregonensis DBVPG 6149 | 14.81 ± 4.46 | 6.69 ± 1.22 | 0.57 ± 0.08 | 7.40 ± 3.19 | 0 | 0.15 ± 0.13 | 0 |
C. sake DBVPG 6162 | 0.05 ± 0.05 | 0.05 ± 0.04 | 0.01 ± 0.01 | 0 | 0 | 0 | 0 |
C. freyschussii DBVPG 6208 | 1.09 ± 0.26 | 1.00 ± 0.23 | 0.01 ± 0.01 | 0.07 ± 0.02 | 0 | 0 | 0 |
W. canadensis DBVPG 6211 | 2.05 ± 0.24 | 0.36 ± 0.12 | 0 | 1.32 ± 0.15 | 0.13 ± 0.22 | 0.24 ± 0.22 | 0 |
Cr. albidus DBVPG 6237 | 0.74 ± 1.08 | 0.43 ± 0.56 | 0.30 ± 0.53 | 0 | 0 | 0 | 0 |
Cr. terreus DBVPG 6242 | 7.38 ± 12.43 | 7.24 ± 12.21 | 0.14 ± 0.22 | 0 | 0 | 0 | 0 |
V. polyspora DBVPG 6243 | 13.45 ± 17.77 | 13.35 ± 17.65 | 0.10 ± 0.13 | 0 | 0 | 0 | 0 |
K. lodderae DBVPG 6308 | 0.17 ± 0.04 | 0.17 ± 0.04 | 0 | 0 | 0 | 0 | 0 |
L. amylophila DBVPG 6346 | 7.79 ± 4.91 | 7.37 ± 4.64 | 0.42 ± 0.28 | 0 | 0 | 0 | 0 |
K. exigua DBVPG 6469 | 4.29 ± 0.83 | 2.87 ± 0.29 | 0.22 ± 0.04 | 1.20 ± 0.53 | 0 | 0 | 0 |
Cr.s terreus DBVPG 6685 | 0.94 ± 0.42 | 0.90 ± 0.40 | 0.05 ± 0.02 | 0 | 0 | 0 | 0 |
Kaz. spencerorum DBVPG 6746 | 11.38 ± 16.72 | 10.85 ± 16.23 | 0.46 ± 0.54 | 0.06 ± 0.10 | 0 | 0 | 0 |
Kaz. spencerorum DBVPG 6748 | 4.43 ± 0.28 | 3.98 ± 0.25 | 0.46 ± 0.03 | 0 | 0 | 0 | 0 |
H. guilliermondii DBVPG 6790 | 63.59 ± 15.44 | 62.11 ± 14.99 | 1.49 ± 0.45 | 0 | 0 | 0 | 0 |
H. occidentalis DBVPG 6798 | 6.26 ± 1.45 | 4.90 ± 1.05 | 0.41 ± 0.09 | 0.95 ± 0.33 | 0 | 0 | 0 |
C. shehatae DBVPG 6850 | 16.93 ± 3.20 | 7.59 ± 0.24 | 0.63 ± 0.02 | 8.71 ± 2.99 | 0 | 0 | 0 |
K. marxianus DBVPG 6854 | 0.24 ± 0.04 | 0.24 ± 0.04 | 0 | 0 | 0 | 0 | 0 |
Kaz. africana DBVPG 6934 | 1.44 ± 0.12 | 1.28 ± 0.11 | 0.16 ± 0.01 | 0 | 0 | 0 | 0 |
N. bacillisporus DBVPG 6945 | 0.33 ± 0.01 | 0.30 ± 0.02 | 0.03 ± 0.00 | 0 | 0 | 0 | 0 |
N. bacillisporus DBVPG 6962 | 1.27 ± 0.80 | 1.16 ± 0.70 | 0.10 ± 0.11 | 0 | 0 | 0 | 0 |
D. nepalensis DBVPG 7123 | 0.05 ± 0.01 | 0.05 ± 0.01 | 0 | 0 | 0 | 0 | 0 |
D. coudertii DBVPG 7124 | 0.020± 0.04 | 0.02 ± 0.04 | 0 | 0 | 0 | 0 | 0 |
Kaz. naganishii DBVPG 7133 | 12.25 ± 10.56 | 11.68 ± 10.26 | 0.57 ± 0.30 | 0 | 0 | 0 | 0 |
Strain | Conversion (mol %) | Main products (mol %) | ||
---|---|---|---|---|
5 | 6 | 7 | ||
C. maltosa DBVPG 6021 | 97.86 ± 0.47 | 95.56 ± 1.34 | 0 | 2.29 ± 0.98 |
Cr. gastricus DBVPG 6057 | 78.22 ± 20.3 | 65.64 ± 4.77 | 0 | 2.41 ± 0.15 |
C. oregonensis DBVPG 6149 | 98.82 ± 0.29 | 97.93 ± 0.33 | 0 | 0.89 |
C. sake DBVPG 6162 | 91.36 ± 1.24 | 0 | 74.80 ± 5.25 | 16.56 ± 4.81 |
C. freyschussii DBVPG 6208 | 100 ± 0.00 | 96.58 ± 1.87 | 0 | 4.15 ± 0.65 |
W. canadensis DBVPG 6211 | 52.58 ± 38.44 | 27.62 ± 47. 84 | 1.89 ± 1.68 | 23.07 ± 8.65 |
Cr. albidus DBVPG 6237 | 99.52 ± 0.82 | 2.26 ± 1.96 | 0 | 67.48 ± 38.82 |
Cr. terreus DBVPG 6242 | 38.49 ± 53.49 | 36.27 ± 55.37 | 0.58 ± 0.51 | 1.63 ± 1.49 |
V. polyspora DBVPG 6243 | 83.34 ± 4.43 | 79.06 ± 7.20 | 0 | 1.80 ± 0.78 |
K. lodderae DBVPG 6308 | 87.13 ± 1.79 | 84.20 ± 4.05 | 0 | 0.33 ± 0.19 |
L. amylophila DBVPG 6346 | 99.19 ± 0.17 | 0 | 0 | 82.65 ± 5.30 |
K. exigua DBVPG 6469 | 43.46 ± 37.32 | 17.65 ± 28.62 | 0.14 ± 0.24 | 25.67 ± 44.15 |
Cr.s terreus DBVPG 6685 | 0 | 0 | 0 | 0 |
Kaz. spencerorum DBVPG 6746 | 100 ± 0.00 | 98.68 ± 0.44 | 0 | 1.67 ± 0.39 |
Kaz. spencerorum DBVPG 6748 | 70.81 ± 0.92 | 70.23 ± 1.62 | 0 | 0.70 ± 0.81 |
H. guilliermondii DBVPG 6790 | 99.33 ± 1.16 | 64.20 ± 55.60 | 0 | 27.13 ± 28.72 |
H. occidentalis DBVPG 6798 | 78.53 ± 13.46 | 71.59 ± 12.25 | 0 | 6.10 ± 1.36 |
C. shehatae DBVPG 6850 | 58.50 ± 33.41 | 57.08 ± 32.76 | 0 | 1.46 ± 0.35 |
K. marxianus DBVPG 6854 | 32.65 ± 56.55 | 0 | 0 | 4.61 ± 5.32 |
Kaz. africana DBVPG 6934 | 40.87 ± 13.58 | 35.85 ± 53.03 | 0 | 5.96 ± 2.66 |
N. bacillisporus DBVPG 6945 | 51.64 ± 2.84 | 50.75 ± 2.58 | 0 | 1.15 ± 0.18 |
N. bacillisporus DBVPG 6962 | 97.02 ± 3.27 | 49.78 ± 57.48 | 0 | 11.16 ± 7.85 |
D. nepalensis DBVPG 7123 | 53.97 ± 51.15 | 53.97 ± 51.15 | 0 | 0 |
D. coudertii DBVPG 7124 | 54.41 ± 51.27 | 54.41 ± 51.27 | 0 | 0 |
Kaz. naganishii DBVPG 7133 | 0 | 0 | 0 | 0 |
3. Experimental
3.1. Chemicals and Culture Media
3.2. Yeast Strains
Pecies and Strain | Origin | Location |
---|---|---|
Candida maltosa DBVPG 6021 | Soil | Japan |
Cryptococcus gastricus DBVPG 6057 | Soil | New Zealand |
Candida oregonensis DBVPG 6149 | Frass of Tsuga heterophylla | USA |
Candida sake DBVPG 6162 | Soil | Sweden |
Candida freyschussii DBVPG 6208 | Wood pulp | Sweden |
Wickerhamomyces canadensis DBVPG 6211 | Wood pulp | Sweden |
Cryptococcus albidus DBVPG 6237 | Soil | Hungary |
Cryptococcus terreus DBVPG 6242 | Soil | Buthan |
Vanderwaltozyma polyspora DBVPG 6243 | Soil | South Africa |
Kazachstania lodderae DBVPG 6308 | Soil | South Africa |
Lindnera amylophila DBVPG 6346 | Frass of Pinus taeda | USA |
Kazachstania exigua DBVPG 6469 | Soil | South Africa |
Cryptococcus terreus DBVPG 6685 | Soil | ex USSR |
Kazachstania spencerorum DBVPG 6746 | Soil | South Africa |
Kazachstania spencerorum DBVPG 6748 | Gut of Psychidae larva | South Africa |
Hanseniaspora guilliermondii DBVPG 6790 | Trachea of bee | France |
Hanseniaspora occidentalis DBVPG 6798 | Soil | West Indies |
Candida shehatae DBVPG 6850 | Rain forest drosophilids | Brazil |
Kluyveromyces marxianus DBVPG 6854 | Rain forest drosophilids | Brazil |
Kazachstania africana DBVPG 6934 | Soil | Zimbabwe |
Nakaseomyces bacillisporus DBVPG 6945 | Exudate of Quercus emoryi | USA |
Nakaseomyces bacillisporus DBVPG 6962 | Exudate of Quercus emoryi | USA |
Debaryomyces nepalensis DBVPG 7123 | Soil | Nepal |
Debaryomyces coudertii DBVPG 7124 | Dropping of Aptenodytes patgonica | France |
Kazachstania naganishii DBVPG 7133 | Decaying leaves | Japan |
3.3. Preparation of Lyophilized Whole-Cells Biocatalysts
3.4. Bioconversion Reactions
3.5. SPME and GC-MS Analyses
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Share and Cite
Goretti, M.; Turchetti, B.; Cramarossa, M.R.; Forti, L.; Buzzini, P. Production of Flavours and Fragrances via Bioreduction of (4R)-(-)-Carvone and (1R)-(-)-Myrtenal by Non-Conventional Yeast Whole-Cells. Molecules 2013, 18, 5736-5748. https://doi.org/10.3390/molecules18055736
Goretti M, Turchetti B, Cramarossa MR, Forti L, Buzzini P. Production of Flavours and Fragrances via Bioreduction of (4R)-(-)-Carvone and (1R)-(-)-Myrtenal by Non-Conventional Yeast Whole-Cells. Molecules. 2013; 18(5):5736-5748. https://doi.org/10.3390/molecules18055736
Chicago/Turabian StyleGoretti, Marta, Benedetta Turchetti, Maria Rita Cramarossa, Luca Forti, and Pietro Buzzini. 2013. "Production of Flavours and Fragrances via Bioreduction of (4R)-(-)-Carvone and (1R)-(-)-Myrtenal by Non-Conventional Yeast Whole-Cells" Molecules 18, no. 5: 5736-5748. https://doi.org/10.3390/molecules18055736