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Molecules 2013, 18(3), 3467-3478; doi:10.3390/molecules18033467
Article

The Ameliorative Effects of L-2-Oxothiazolidine-4-Carboxylate on Acetaminophen-Induced Hepatotoxicity in Mice

1,2,†
, 3,†
, 2
, 4
 and 4,5,*
1 Department of Radiological Sciences, Jeonju University, Jeonju 560-759, Korea 2 Department of Physiology, School of Medicine, Chonbuk National University, Jeonju 561-803, Korea 3 Department of Oriental Pharmaceutical Development, Nambu University, Gwangju 506-706, Korea 4 Ato Q&A Incorperation, Jeonju 560-759, Korea 5 Department of Health & Science, Jeonju University, Jeonju 560-759, Korea These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 23 January 2013 / Revised: 31 January 2013 / Accepted: 14 March 2013 / Published: 18 March 2013
(This article belongs to the Special Issue Antioxidants 2012)
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Abstract

The aim of the study was to investigate the ameliorative effects and the mechanism of action of L-2-oxothiazolidine-4-carboxylate (OTC) on acetaminophen (APAP)-induced hepatotoxicity in mice. Mice were randomly divided into six groups: normal control group, APAP only treated group, APAP + 25 mg/kg OTC, APAP + 50 mg/kg OTC, APAP + 100 mg/kg OTC, and APAP + 100 mg/kg N-acetylcysteine (NAC) as a reference control group. OTC treatment significantly reduced serum alanine aminotransferase and aspartate aminotransferase levels in a dose dependent manner. OTC treatment was markedly increased glutathione (GSH) production and glutathione peroxidase (GSH-px) activity in a dose dependent manner. The contents of malondialdehyde and 4-hydroxynonenal in liver tissues were significantly decreased by administration of OTC and the inhibitory effect of OTC was similar to that of NAC. Moreover, OTC treatment on APAP-induced hepatotoxicity significantly reduced the formation of nitrotyrosin and terminal deoxynucleotidyl transferase dUTP nick end labeling positive areas of liver tissues in a dose dependent manner. Furthermore, the activity of caspase-3 in liver tissues was reduced by administration of OTC in a dose dependent manner. The ameliorative effects of OTC on APAP-induced liver damage in mice was similar to that of NAC. These results suggest that OTC has ameliorative effects on APAP-induced hepatotoxicity in mice through anti-oxidative stress and anti-apoptotic processes.
Keywords: L-2-oxothiazolidine-4-carboxylate; acetaminophen-induced hepatotoxicity; apoptosis; oxidative stress; antioxidant L-2-oxothiazolidine-4-carboxylate; acetaminophen-induced hepatotoxicity; apoptosis; oxidative stress; antioxidant
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Choi, J.; Park, K.-H.; Kim, S.Z.; Shin, J.H.; Jang, S.-I. The Ameliorative Effects of L-2-Oxothiazolidine-4-Carboxylate on Acetaminophen-Induced Hepatotoxicity in Mice. Molecules 2013, 18, 3467-3478.

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