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Molecules 2013, 18(3), 2528-2538; doi:10.3390/molecules18032528
Article

Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension Culture

1,2,3,* , 2
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Received: 13 December 2012; in revised form: 29 January 2013 / Accepted: 16 February 2013 / Published: 26 February 2013
(This article belongs to the Special Issue Bioassay-Guided Isolation of Natural Products)
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Abstract: This present work describes the application of liquid chromatograpy-solid phase extraction-nuclear magnetic resonance spectroscopy to analyse Alternaria alternata crude extracts. Altenusin (1), alternariol (2), 3'-hydroxyalternariol monomethyl ether (3), and alternariol monomethyl ether (4), were separated and identified. High-resolution mass spectrometry confirmed the proposed structures. The cytotoxic effects of these compounds towards plants were determined using soybean (Glycine max) cell cultures as a model. EC50 values which range from 0.11 (±0.02) to 4.69 (±0.47) μM showed the high cytotoxicity of these compounds.
Keywords: LC-NMR; LC-SPE-NMR; mass spectrometry; alternariol; Alternaria; soybean; Glycine max; cell cultures; cytotoxicity LC-NMR; LC-SPE-NMR; mass spectrometry; alternariol; Alternaria; soybean; Glycine max; cell cultures; cytotoxicity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

de Souza, G.D.; Mithöfer, A.; Daolio, C.; Schneider, B.; Rodrigues-Filho, E. Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension Culture. Molecules 2013, 18, 2528-2538.

AMA Style

de Souza GD, Mithöfer A, Daolio C, Schneider B, Rodrigues-Filho E. Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension Culture. Molecules. 2013; 18(3):2528-2538.

Chicago/Turabian Style

de Souza, Gezimar D.; Mithöfer, Axel; Daolio, Cristina; Schneider, Bernd; Rodrigues-Filho, Edson. 2013. "Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension Culture." Molecules 18, no. 3: 2528-2538.



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