Molecules 2013, 18(3), 2539-2548; doi:10.3390/molecules18032539
Article

Methyl Antcinate A Suppresses the Population of Cancer Stem-Like Cells in MCF7 Human Breast Cancer Cell Line

1 School of Dentistry, Chung Shan Medical University, Taichung 402, Taiwan 2 Department of Dentistry, Chung Shan Medical University Hospital, Taichung 402, Taiwan 3 Department of Biomedical Sciences, Chung Shan Medical University, Taichung 402, Taiwan 4 Institute of Oral Science, Chung Shan Medical University, Taichung 402, Taiwan 5 Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan 6 Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan 7 Institute of Biochemical Sciences and Technology, Chaoyang University of Technology, Taichung 413, Taiwan 8 Department of Medical Research, Chung Shan Medical University Hospital, Taichung 402, Taiwan
* Author to whom correspondence should be addressed.
Received: 12 December 2012; in revised form: 22 January 2013 / Accepted: 19 February 2013 / Published: 26 February 2013
(This article belongs to the Special Issue Triterpenes and Triterpenoids)
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Abstract: Methyl antcinate A (MAA) is an ergostane-type triterpenoid extracted from the fruiting bodies of Antrodia camphorate that has been reported to be a cytotoxic agent towards some types of cancer cells, such as oral cancer and liver cancer. Cancer stem cells (CSCs) are a particular population within cancer cells which are responsible for tumor initiation, drug resistance and metastasis and targeting CSCs is an emerging area in cancer therapy. In this study, we examine the effect of MAA on cancer stem-like cells in the MCF7 human breast cancer cell line. Although MAA displayed very low cytotoxic effect towards MCF7 under normal culture conditions, it did show good inhibitory effects on the self-renewal capability which was examined by mammosphere culture including primary and secondary sphere. MAA also inhibited cell migration ability of MCF7 sphere cells. By western blot analysis, MAA was shown to suppress the expression of heat shock protein 27 and increase the expression of IkBα and p53. In conclusion, our data demonstrate that MAA has anti-CSC activity and is worthy of future development of potent anticancer agents.
Keywords: methyl antcinate A; breast cancer stem-like cells; p53; Hsp27; IkBα

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MDPI and ACS Style

Peng, C.-Y.; Fong, P.-C.; Yu, C.-C.; Tsai, W.-C.; Tzeng, Y.-M.; Chang, W.-W. Methyl Antcinate A Suppresses the Population of Cancer Stem-Like Cells in MCF7 Human Breast Cancer Cell Line. Molecules 2013, 18, 2539-2548.

AMA Style

Peng C-Y, Fong P-C, Yu C-C, Tsai W-C, Tzeng Y-M, Chang W-W. Methyl Antcinate A Suppresses the Population of Cancer Stem-Like Cells in MCF7 Human Breast Cancer Cell Line. Molecules. 2013; 18(3):2539-2548.

Chicago/Turabian Style

Peng, Chih-Yu; Fong, Pin-Chung; Yu, Cheng-Chia; Tsai, Wan-Chi; Tzeng, Yew-Min; Chang, Wen-Wei. 2013. "Methyl Antcinate A Suppresses the Population of Cancer Stem-Like Cells in MCF7 Human Breast Cancer Cell Line." Molecules 18, no. 3: 2539-2548.

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