Next Article in Journal
Synthesis, Structure and Cytotoxic Activity of New Acetylenic Derivatives of Betulin
Next Article in Special Issue
Vasodilator Compounds Derived from Plants and Their Mechanisms of Action
Previous Article in Journal
Synthesis and Biological Evaluation of Thieno[3,2-d]- pyrimidinones, Thieno[3,2-d]pyrimidines and Quinazolinones: Conformationally Restricted 17b-Hydroxysteroid Dehydrogenase Type 2 (17b-HSD2) Inhibitors
Previous Article in Special Issue
Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension Culture
Molecules 2013, 18(4), 4510-4525; doi:10.3390/molecules18044510
Article

The Use of Bio-Guided Fractionation to Explore the Use of Leftover Biomass in Dutch Flower Bulb Production as Allelochemicals against Weeds

1,2,3,* , 1,4
, 3
, 1
 and 3
Received: 28 February 2013; in revised form: 28 March 2013 / Accepted: 12 April 2013 / Published: 17 April 2013
(This article belongs to the Special Issue Bioassay-Guided Isolation of Natural Products)
Download PDF [649 KB, uploaded 18 June 2014]
Abstract: A major problem in flower bulb cultivation is weed control. Synthetic herbicides are mainly used, although they cause a range of problems, and integrated weed control through application of naturally occurring allelochemicals would be highly desirable. Flower bulb production creates large amounts of leftover biomass. Utilizing this source for weed control may provide new applications of the bulb crops. We therefore screened 33 flower bulb extracts for allelochemical activity against weeds. Several methanol and chloroform extracts were observed to inhibit germination and growth of Senecio vulgaris L. and Lolium perenne L., as representatives of di- and mono-cotyledonous weeds, respectively. Narciclasine was identified as the bioactive compound in Narcissus. The extract of Amaryllis belladonna L. was equally active, but did not contain any narciclasine. Bioassay-guided fractionation of the A. belladonna extract resulted in the identification of lycorine as the bio-active compound. The IC50 measured for radicle growth inhibition was 0.10 µM for narciclasine and 0.93 µM for lycorine, compared to 0.11 mM of chlorpropham, a synthetic herbicide. Therefore, the leftover biomass from the spring bulb industry represents an interesting potential source for promising allelochemicals for further studies on weed growth inhibition.
Keywords: allelochemicals; bio-guided fractionation; flower bulbs; alkaloids; weeds allelochemicals; bio-guided fractionation; flower bulbs; alkaloids; weeds
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Wahyuni, D.S.C.; van der Kooy, F.; Klinkhamer, P.G.L.; Verpoorte, R.; Leiss, K. The Use of Bio-Guided Fractionation to Explore the Use of Leftover Biomass in Dutch Flower Bulb Production as Allelochemicals against Weeds. Molecules 2013, 18, 4510-4525.

AMA Style

Wahyuni DSC, van der Kooy F, Klinkhamer PGL, Verpoorte R, Leiss K. The Use of Bio-Guided Fractionation to Explore the Use of Leftover Biomass in Dutch Flower Bulb Production as Allelochemicals against Weeds. Molecules. 2013; 18(4):4510-4525.

Chicago/Turabian Style

Wahyuni, Dinar S.C.; van der Kooy, Frank; Klinkhamer, Peter G.L.; Verpoorte, Rob; Leiss, Kirsten. 2013. "The Use of Bio-Guided Fractionation to Explore the Use of Leftover Biomass in Dutch Flower Bulb Production as Allelochemicals against Weeds." Molecules 18, no. 4: 4510-4525.


Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert