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Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots
Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic
Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, Brno CZ-616 00, Czech Republic
* Author to whom correspondence should be addressed.
Received: 10 October 2013; in revised form: 25 November 2013 / Accepted: 5 December 2013 / Published: 13 December 2013
Abstract: In this study we describe a beads-based assay for rapid, sensitive and specific isolation and detection of influenza vaccine hemagglutinin (HA). Amplification of the hemagglutinin signal resulted from binding of an electrochemical label as quantum dots (QDs). For detection of the metal and protein part of the resulting HA-CdTe complex, two differential pulse voltammetric methods were used. The procedure includes automated robotic isolation and electrochemical analysis of the isolated product. The isolation procedure was based on the binding of paramagnetic particles (MPs) with glycan (Gly), where glycan was used as the specific receptor for linkage of the QD-labeled hemagglutinin.
Keywords: influenza hemagglutinin; electrochemical detection; paramagnetic particles; glycan; quantum dot labeling
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Krejcova, L.; Nejdl, L.; Hynek, D.; Krizkova, S.; Kopel, P.; Adam, V.; Kizek, R. Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots. Molecules 2013, 18, 15573-15586.
Krejcova L, Nejdl L, Hynek D, Krizkova S, Kopel P, Adam V, Kizek R. Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots. Molecules. 2013; 18(12):15573-15586.
Krejcova, Ludmila; Nejdl, Lukas; Hynek, David; Krizkova, Sona; Kopel, Pavel; Adam, Vojtech; Kizek, Rene. 2013. "Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots." Molecules 18, no. 12: 15573-15586.