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Molecules 2013, 18(1), 482-519; doi:10.3390/molecules18010482
Review

Access to Any Site Directed Stable Isotope (2H, 13C, 15N, 17O and 18O) in Genetically Encoded Amino Acids

 and *
Received: 25 October 2012; in revised form: 10 December 2012 / Accepted: 24 December 2012 / Published: 2 January 2013
(This article belongs to the Special Issue Chemical Protein and Peptide Synthesis)
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Abstract: Proteins and peptides play a preeminent role in the processes of living cells. The only way to study structure-function relationships of a protein at the atomic level without any perturbation is by using non-invasive isotope sensitive techniques with site-directed stable isotope incorporation at a predetermined amino acid residue in the protein chain. The method can be extended to study the protein chain tagged with stable isotope enriched amino acid residues at any position or combinations of positions in the system. In order to access these studies synthetic methods to prepare any possible isotopologue and isotopomer of the 22 genetically encoded amino acids have to be available. In this paper the synthetic schemes and the stable isotope enriched building blocks that are available via commercially available stable isotope enriched starting materials are described.
Keywords: amino acids; isotope labelling; [5-13C]-leucine; [4-13C]-valine; 13C or 15N-enriched L-lysine; [18O]-benzylchloromethyl ether; [13C]-benzonitrile amino acids; isotope labelling; [5-13C]-leucine; [4-13C]-valine; 13C or 15N-enriched L-lysine; [18O]-benzylchloromethyl ether; [13C]-benzonitrile
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Dawadi, P.B.S.; Lugtenburg, J. Access to Any Site Directed Stable Isotope (2H, 13C, 15N, 17O and 18O) in Genetically Encoded Amino Acids. Molecules 2013, 18, 482-519.

AMA Style

Dawadi PBS, Lugtenburg J. Access to Any Site Directed Stable Isotope (2H, 13C, 15N, 17O and 18O) in Genetically Encoded Amino Acids. Molecules. 2013; 18(1):482-519.

Chicago/Turabian Style

Dawadi, Prativa B.S.; Lugtenburg, Johan. 2013. "Access to Any Site Directed Stable Isotope (2H, 13C, 15N, 17O and 18O) in Genetically Encoded Amino Acids." Molecules 18, no. 1: 482-519.


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