Molecules 2013, 18(1), 440-465; doi:10.3390/molecules18010440
Review

Semi-Synthesis of Labeled Proteins for Spectroscopic Applications

Lucia De Rosa 1 email, Anna Russomanno 1 email, Alessandra Romanelli 2 email and Luca Domenico D'Andrea 1,* email
1 Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16, Napoli 80134, Italy 2 Dipartimento delle Scienze Biologiche, Università di Napoli "Federico II", Via Mezzocannone 16, Napoli 80134, Italy
* Author to whom correspondence should be addressed.
Received: 28 November 2012; in revised form: 21 December 2012 / Accepted: 24 December 2012 / Published: 2 January 2013
(This article belongs to the Special Issue Chemical Protein and Peptide Synthesis)
PDF Full-text Download PDF Full-Text [1437 KB, Updated Version, uploaded 7 January 2013 16:05 CET]
The original version is still available [1437 KB, uploaded 2 January 2013 11:22 CET]
Abstract: Since the introduction of SPPS by Merrifield in the 60s, peptide chemists have considered the possibility of preparing large proteins. The introduction of native chemical ligation in the 90s and then of expressed protein ligation have opened the way to the preparation of synthetic proteins without size limitations. This review focuses on semi-synthetic strategies useful to prepare proteins decorated with spectroscopic probes, like fluorescent labels and stable isotopes, and their biophysical applications. We show that expressed protein ligation, combining the advantages of organic chemistry with the easy and size limitless recombinant protein expression, is an excellent strategy for the chemical synthesis of labeled proteins, enabling a single protein to be functionalized at one or even more distinct positions with different probes.
Keywords: expressed protein ligation; intein; Förster resonance energy transfer; segmental labeling; NMR; protein labeling

Article Statistics

Click here to load and display the download statistics.

Cite This Article

MDPI and ACS Style

De Rosa, L.; Russomanno, A.; Romanelli, A.; D'Andrea, L.D. Semi-Synthesis of Labeled Proteins for Spectroscopic Applications. Molecules 2013, 18, 440-465.

AMA Style

De Rosa L, Russomanno A, Romanelli A, D'Andrea LD. Semi-Synthesis of Labeled Proteins for Spectroscopic Applications. Molecules. 2013; 18(1):440-465.

Chicago/Turabian Style

De Rosa, Lucia; Russomanno, Anna; Romanelli, Alessandra; D'Andrea, Luca D. 2013. "Semi-Synthesis of Labeled Proteins for Spectroscopic Applications." Molecules 18, no. 1: 440-465.

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert