Molecules 2013, 18(1), 520-534; doi:10.3390/molecules18010520
Article

Antioxidant Properties of cis-Z,Z'-3a.7a',7a.3a'-Dihydroxy-ligustilide on Human Umbilical Vein Endothelial Cells in Vitro

Weidong Li 1,2 , Yu Wu 1,2 , Xuedong Liu 1 , Cuiping Yan 1,2 , Dan Liu 3 , Yang Pan 1 , Guangming Yang 1,2 , Fangzhou Yin 1,2 , Zebin Weng 1,2 , Ding Zhao 1 , Zhipeng Chen 1,2,* email and Baochang Cai 1,2,4,* email
1 College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China 2 Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210046, China 3 College of Pharmacy, China Pharmaceutical University, Nanjing 210009, China 4 Department of R&D, Nanjing Haichang Chinese Medicine Group Corporation, Nanjing 210061, China
* Authors to whom correspondence should be addressed.
Received: 16 November 2012; in revised form: 14 December 2012 / Accepted: 18 December 2012 / Published: 2 January 2013
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Abstract: A new chemical component, cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide, was isolated from Angelica sinensis and its structure elucidated from its NMR and MS spectra and confirmed by X-ray single crystal diffraction analysis. We also explored the antioxidative properties of cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide on human umbilical vein endothelial cells (HUVECs) against injuries induced by hydrogen peroxide (H2O2) using an MTT assay and flow cytometry analysis. In addition, the activities of superoxide dismutase (SOD), malondialdehyde (MDA), lactate dehydrogenase (LDH), nitric oxide (NO) and reactive oxygen species (ROS) were determined. We found that cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide increased the viability of HUVECs injured by H2O2 in a dose-dependent manner, reduced the apoptosis of HUVEC, and enhanced HUVEC proliferation. Our results demonstrated the remarkable in vitro antioxidative activities of this compound, indicating that it could be a potential antioxidant with protective effects against H2O2-induced HUVEC injuries.
Keywords: cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide; antioxidant; human umbilical vein endothelial cell; cell cycle

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Cite This Article

MDPI and ACS Style

Li, W.; Wu, Y.; Liu, X.; Yan, C.; Liu, D.; Pan, Y.; Yang, G.; Yin, F.; Weng, Z.; Zhao, D.; Chen, Z.; Cai, B. Antioxidant Properties of cis-Z,Z'-3a.7a',7a.3a'-Dihydroxy-ligustilide on Human Umbilical Vein Endothelial Cells in Vitro. Molecules 2013, 18, 520-534.

AMA Style

Li W, Wu Y, Liu X, Yan C, Liu D, Pan Y, Yang G, Yin F, Weng Z, Zhao D, Chen Z, Cai B. Antioxidant Properties of cis-Z,Z'-3a.7a',7a.3a'-Dihydroxy-ligustilide on Human Umbilical Vein Endothelial Cells in Vitro. Molecules. 2013; 18(1):520-534.

Chicago/Turabian Style

Li, Weidong; Wu, Yu; Liu, Xuedong; Yan, Cuiping; Liu, Dan; Pan, Yang; Yang, Guangming; Yin, Fangzhou; Weng, Zebin; Zhao, Ding; Chen, Zhipeng; Cai, Baochang. 2013. "Antioxidant Properties of cis-Z,Z'-3a.7a',7a.3a'-Dihydroxy-ligustilide on Human Umbilical Vein Endothelial Cells in Vitro." Molecules 18, no. 1: 520-534.

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