MALDI-TOF MS Analysis of Native and Permethylated or Benzimidazole-Derivatized Polysaccharides
AbstractMALDI-TOF MS provides rapid and sensitive analyses of larger biomolecules. However, MS analyses of polysaccharide have been reported to have lower sensitivity compared to peptides and proteins. Here, we investigated some polysaccharides chemically derivatized by permethylation and ortho-phenylene diamine (OPD) tagging. Methylated glycan is obviously able to improve the sensitivity for mass spectrometry detection. Oxidative condensation by UV-activation tagging to saccharides by OPD and peptide-OPD also improve the sensitivity of MALDI-TOF MS analyses. Polysaccharides including dextran, glucomannan, arabinoxylan, arabinogalactan and beta-1,3-glucan, isolated from nutritional supplements of Ganoderma lucidum and Saccharomyces pastorianus were measured using MALDI-TOF MS with 2,5-dihydroxybenzoic acid (2,5-DHB) as the matrix. These glycans were also derivatized to methylated and benzimidazole-tagged glycans by chemical transformation for molecular weight analysis. The derivatized polysaccharides showed excellent MALDI-TOF MS signal enhancement in the molecular weight range from 1 to 5 kDa. Here, we demonstrate an efficient method to give glycan-benzimidazole (glycan-BIM) derivatives for polysaccharide determination in MALDI-TOF MS. Therefore, permethylated or benzimidazole-derivatized polysaccharides provide a new option for polysaccharide analysis using MALDI-TOF MS.
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Hung, W.-T.; Wang, S.-H.; Chen, Y.-T.; Yu, H.-M.; Chen, C.-H.; Yang, W.-B. MALDI-TOF MS Analysis of Native and Permethylated or Benzimidazole-Derivatized Polysaccharides. Molecules 2012, 17, 4950-4961.
Hung W-T, Wang S-H, Chen Y-T, Yu H-M, Chen C-H, Yang W-B. MALDI-TOF MS Analysis of Native and Permethylated or Benzimidazole-Derivatized Polysaccharides. Molecules. 2012; 17(5):4950-4961.Chicago/Turabian Style
Hung, Wei-Ting; Wang, Shwu-Huey; Chen, Yi-Ting; Yu, Hui-Ming; Chen, Chung-Hsuan; Yang, Wen-Bin. 2012. "MALDI-TOF MS Analysis of Native and Permethylated or Benzimidazole-Derivatized Polysaccharides." Molecules 17, no. 5: 4950-4961.