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Molecules 2012, 17(5), 4950-4961; doi:10.3390/molecules17054950

MALDI-TOF MS Analysis of Native and Permethylated or Benzimidazole-Derivatized Polysaccharides

Genomics Research Center, Academia Sinica, 128, Academia Road Sec. 2, Taipei 115, Taiwan
Core Facility Center, Office of Research and Development, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 20 December 2011 / Revised: 6 April 2012 / Accepted: 20 April 2012 / Published: 30 April 2012
(This article belongs to the Special Issue Natural Polysaccharides: Chemistry, Bioactivity and Analysis)
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MALDI-TOF MS provides rapid and sensitive analyses of larger biomolecules. However, MS analyses of polysaccharide have been reported to have lower sensitivity compared to peptides and proteins. Here, we investigated some polysaccharides chemically derivatized by permethylation and ortho-phenylene diamine (OPD) tagging. Methylated glycan is obviously able to improve the sensitivity for mass spectrometry detection. Oxidative condensation by UV-activation tagging to saccharides by OPD and peptide-OPD also improve the sensitivity of MALDI-TOF MS analyses. Polysaccharides including dextran, glucomannan, arabinoxylan, arabinogalactan and beta-1,3-glucan, isolated from nutritional supplements of Ganoderma lucidum and Saccharomyces pastorianus were measured using MALDI-TOF MS with 2,5-dihydroxybenzoic acid (2,5-DHB) as the matrix. These glycans were also derivatized to methylated and benzimidazole-tagged glycans by chemical transformation for molecular weight analysis. The derivatized polysaccharides showed excellent MALDI-TOF MS signal enhancement in the molecular weight range from 1 to 5 kDa. Here, we demonstrate an efficient method to give glycan-benzimidazole (glycan-BIM) derivatives for polysaccharide determination in MALDI-TOF MS. Therefore, permethylated or benzimidazole-derivatized polysaccharides provide a new option for polysaccharide analysis using MALDI-TOF MS.
Keywords: polysaccharides; MALDI-TOF MS; permethylation; benzimidazole; 2,5-DHB polysaccharides; MALDI-TOF MS; permethylation; benzimidazole; 2,5-DHB
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Hung, W.-T.; Wang, S.-H.; Chen, Y.-T.; Yu, H.-M.; Chen, C.-H.; Yang, W.-B. MALDI-TOF MS Analysis of Native and Permethylated or Benzimidazole-Derivatized Polysaccharides. Molecules 2012, 17, 4950-4961.

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