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Immunostimulatory Effect of Laminarin on RAW 264.7 Mouse Macrophages
College of Korean Medicine, Gachon University, Seongnam 461-701, Korea
* Authors to whom correspondence should be addressed.
Received: 1 March 2012; in revised form: 25 April 2012 / Accepted: 3 May 2012 / Published: 8 May 2012
Abstract: This study investigated the immunostimulatory effects of laminarin with respect to inflammatory mediators such as hydrogen peroxide, calcium, nitric oxide, various cytokines, transcription factors, and immune response gene in RAW 264.7 mouse macrophages. Laminarin did not reduce the cell proliferation of RAW 264.7 mouse macrophages at concentrations up to 500 µg/mL. Laminarin significantly increased the release of hydrogen peroxide, calcium, nitric oxide, monocyte chemotactic protein-1, vascular endothelial growth factor, leukemia inhibitory factor, and granulocyte-colony stimulating factor with enhancing expression of Signal Transducer and Activator of Transcription 1 (STAT1), STAT3, c-Jun, c-Fos, and cyclooxygenase-2 mRNA in RAW 264.7 cells. The results suggest that laminarin has immunostimulatory properties, strengthening immune reactions via the transcription factor pathway in macrophages.
Keywords: laminarin; macrophage; immunostimulatory; cytokine; nitric oxide; hydrogen peroxide; calcium; transcription factor
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MDPI and ACS Style
Lee, J.Y.; Kim, Y.-J.; Kim, H.J.; Kim, Y.-S.; Park, W. Immunostimulatory Effect of Laminarin on RAW 264.7 Mouse Macrophages. Molecules 2012, 17, 5404-5411.
Lee JY, Kim Y-J, Kim HJ, Kim Y-S, Park W. Immunostimulatory Effect of Laminarin on RAW 264.7 Mouse Macrophages. Molecules. 2012; 17(5):5404-5411.
Lee, Ji Young; Kim, Young-Jin; Kim, Hyun Joo; Kim, Yoon-Sang; Park, Wansu. 2012. "Immunostimulatory Effect of Laminarin on RAW 264.7 Mouse Macrophages." Molecules 17, no. 5: 5404-5411.