Next Article in Journal
Evaluation of Antioxidant and Immunity Function of Tetramethylpyrazine Phosphate Tablets in Vivo
Next Article in Special Issue
Influence of Chemical Extraction Conditions on the Physicochemical and Functional Properties of Polysaccharide Gum from Durian (Durio zibethinus) Seed
Previous Article in Journal
Two New Cyanogenic Glucosides from the Leaves of Hydrangea macrophylla
Previous Article in Special Issue
MALDI-TOF MS Analysis of Native and Permethylated or Benzimidazole-Derivatized Polysaccharides
Molecules 2012, 17(5), 5404-5411; doi:10.3390/molecules17055404
Communication

Immunostimulatory Effect of Laminarin on RAW 264.7 Mouse Macrophages

,
,
,
*  and *
Received: 1 March 2012 / Revised: 25 April 2012 / Accepted: 3 May 2012 / Published: 8 May 2012
(This article belongs to the Special Issue Natural Polysaccharides: Chemistry, Bioactivity and Analysis)
Download PDF [843 KB, uploaded 18 June 2014]

Abstract

This study investigated the immunostimulatory effects of laminarin with respect to inflammatory mediators such as hydrogen peroxide, calcium, nitric oxide, various cytokines, transcription factors, and immune response gene in RAW 264.7 mouse macrophages. Laminarin did not reduce the cell proliferation of RAW 264.7 mouse macrophages at concentrations up to 500 µg/mL. Laminarin significantly increased the release of hydrogen peroxide, calcium, nitric oxide, monocyte chemotactic protein-1, vascular endothelial growth factor, leukemia inhibitory factor, and granulocyte-colony stimulating factor with enhancing expression of Signal Transducer and Activator of Transcription 1 (STAT1), STAT3, c-Jun, c-Fos, and cyclooxygenase-2 mRNA in RAW 264.7 cells. The results suggest that laminarin has immunostimulatory properties, strengthening immune reactions via the transcription factor pathway in macrophages.
Keywords: laminarin; macrophage; immunostimulatory; cytokine; nitric oxide; hydrogen peroxide; calcium; transcription factor laminarin; macrophage; immunostimulatory; cytokine; nitric oxide; hydrogen peroxide; calcium; transcription factor
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote
MDPI and ACS Style

Lee, J.Y.; Kim, Y.-J.; Kim, H.J.; Kim, Y.-S.; Park, W. Immunostimulatory Effect of Laminarin on RAW 264.7 Mouse Macrophages. Molecules 2012, 17, 5404-5411.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

Cited By

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert