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Molecules 2012, 17(5), 5404-5411; doi:10.3390/molecules17055404

Immunostimulatory Effect of Laminarin on RAW 264.7 Mouse Macrophages

College of Korean Medicine, Gachon University, Seongnam 461-701, Korea
Authors to whom correspondence should be addressed.
Received: 1 March 2012 / Revised: 25 April 2012 / Accepted: 3 May 2012 / Published: 8 May 2012
(This article belongs to the Special Issue Natural Polysaccharides: Chemistry, Bioactivity and Analysis)
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This study investigated the immunostimulatory effects of laminarin with respect to inflammatory mediators such as hydrogen peroxide, calcium, nitric oxide, various cytokines, transcription factors, and immune response gene in RAW 264.7 mouse macrophages. Laminarin did not reduce the cell proliferation of RAW 264.7 mouse macrophages at concentrations up to 500 µg/mL. Laminarin significantly increased the release of hydrogen peroxide, calcium, nitric oxide, monocyte chemotactic protein-1, vascular endothelial growth factor, leukemia inhibitory factor, and granulocyte-colony stimulating factor with enhancing expression of Signal Transducer and Activator of Transcription 1 (STAT1), STAT3, c-Jun, c-Fos, and cyclooxygenase-2 mRNA in RAW 264.7 cells. The results suggest that laminarin has immunostimulatory properties, strengthening immune reactions via the transcription factor pathway in macrophages.
Keywords: laminarin; macrophage; immunostimulatory; cytokine; nitric oxide; hydrogen peroxide; calcium; transcription factor laminarin; macrophage; immunostimulatory; cytokine; nitric oxide; hydrogen peroxide; calcium; transcription factor
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Lee, J.Y.; Kim, Y.-J.; Kim, H.J.; Kim, Y.-S.; Park, W. Immunostimulatory Effect of Laminarin on RAW 264.7 Mouse Macrophages. Molecules 2012, 17, 5404-5411.

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