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Lambda-Display: A Powerful Tool for Antigen Discovery
Sigma-tau Industrie Farmaceutiche Riunite S.p.A., Rome, Italy
* Author to whom correspondence should be addressed.
Received: 12 January 2011; in revised form: 7 April 2011 / Accepted: 12 April 2011 / Published: 13 April 2011
Abstract: Since its introduction in 1985, phage display technology has been successfully used in projects aimed at deciphering biological processes and isolating molecules of practical value in several applications. Bacteriophage lambda, representing a classical molecular cloning and expression system has also been exploited for generating large combinatorial libraries of small peptides and protein domains exposed on its capsid. More recently, lambda display has been consistently and successfully employed for domain mapping, antigen discovery and protein interaction studies or, more generally, in functional genomics. We show here the results obtained by the use of large libraries of cDNA and genomic DNA for the molecular dissection of the human B-cell response against complex pathogens, including protozoan parasites, bacteria and viruses. Moreover, by reviewing the experimental work performed in recent investigations we illustrate the potential of lambda display in the diagnostics field and for identifying antigens useful as targets for vaccine development.
Keywords: bacteriophage lambda; phage display; antigen discovery; diagnostic assay; vaccine
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MDPI and ACS Style
Beghetto, E.; Gargano, N. Lambda-Display: A Powerful Tool for Antigen Discovery. Molecules 2011, 16, 3089-3105.
Beghetto E, Gargano N. Lambda-Display: A Powerful Tool for Antigen Discovery. Molecules. 2011; 16(4):3089-3105.
Beghetto, Elisa; Gargano, Nicola. 2011. "Lambda-Display: A Powerful Tool for Antigen Discovery." Molecules 16, no. 4: 3089-3105.