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Components and Insecticidal Activity against the Maize Weevils of Zanthoxylum schinifolium Fruits and Leaves
State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University, Beijing 100875, China
Department of Entomology, China Agricultural University, Haidian District, Beijing 100094, China
Analytical and Testing Center, Beijing Normal University, Haidian District, Beijing 100875, China
Department of Plant Pathology, China Agricultural University, Haidian District, Beijing 100094, China
* Authors to whom correspondence should be addressed.
Received: 6 March 2011; in revised form: 5 April 2011 / Accepted: 11 April 2011 / Published: 13 April 2011
Abstract: In our screening program for new agrochemicals from Chinese medicinal herbs and wild plants, Zanthoxylum schinifolium essential oils were found to possess strong insecticidal activity against the maize weevil Sitophilus zeamais. The essential oils of Z. schinifolium fruits and leaves were extracted via hydrodistillation and investigated by GC and GC-MS. Estragole (69.52%) was the major compound of the essential oil of fresh fruits, followed by linalool (8.63%) and limonene (4.34%) and 94.33% of the total components were monoterpenoids. The main components of the essential oil of fresh leaves were linalool (12.94%), ar-tumerone (8.95%), limonene (6.45%) and elixene (5.43%) and only 50.62% were monoterpenoids. However, the essential oil from purchased fruits contained linalool (33.42%), limonene (13.66%) and sabinene (5.72%), followed by estragole (4.67%), nerol (4.56%) and 4-terpineol (4.27%). Estragole, linalool and sabinene were separated and purified by silica gel column chromatography and preparative thin layer chromatography, and further identified by means of physicochemical and spectrometric analysis. The essential oil from the fresh fruits (LD50 = 15.93 μg/adult) possessed two times more toxicity to the insects compared with that of fresh leaves (LD50 = 35.31 μg/adult). Estragole, linalool and sabinene exhibited contact activity against S. zeamais with LD50 values of 17.63, 13.90 and 23.98 μg/adult, respectively. The essential oils of Z. schinifolium possessed strong fumigant toxicity against S. zeamais adults with LC50 values of 13.19 mg/L (fresh fruits), 24.04 mg/L (fresh leaves) and 17.63 mg/L (purchased fruits). Estragole, linalool and sabinene also exhibited strong fumigant toxicity against the maize weevils with LC50 values of 14.10, 10.46 and 9.12 mg/L, respectively.
Keywords: Zanthoxylum schinifolium; Sitophilus zeamais; fumigant; contact toxicity; essential oil; estragole; linalool; sabinene
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Wang, C.F.; Yang, K.; Zhang, H.M.; Cao, J.; Fang, R.; Liu, Z.L.; Du, S.S.; Wang, Y.Y.; Deng, Z.W.; Zhou, L. Components and Insecticidal Activity against the Maize Weevils of Zanthoxylum schinifolium Fruits and Leaves. Molecules 2011, 16, 3077-3088.
Wang CF, Yang K, Zhang HM, Cao J, Fang R, Liu ZL, Du SS, Wang YY, Deng ZW, Zhou L. Components and Insecticidal Activity against the Maize Weevils of Zanthoxylum schinifolium Fruits and Leaves. Molecules. 2011; 16(4):3077-3088.
Wang, Cheng Fang; Yang, Kai; Zhang, Hai Ming; Cao, Jie; Fang, Rui; Liu, Zhi Long; Du, Shu Shan; Wang, Yong Yan; Deng, Zhi Wei; Zhou, Ligang. 2011. "Components and Insecticidal Activity against the Maize Weevils of Zanthoxylum schinifolium Fruits and Leaves." Molecules 16, no. 4: 3077-3088.