Next Article in Journal
Role of the Morphology and Polyphosphate in Trichoderma harzianum Related to Cadmium Removal
Next Article in Special Issue
Lambda-Display: A Powerful Tool for Antigen Discovery
Previous Article in Journal
Allobetulin and Its Derivatives: Synthesis and Biological Activity
Previous Article in Special Issue
Diversity of Phage-Displayed Libraries of Peptides during Panning and Amplification
Article Menu

Article Versions

Export Article

Open AccessReview
Molecules 2011, 16(3), 2467-2485; doi:10.3390/molecules16032467

Miniproteins as Phage Display-Scaffolds for Clinical Applications

Clinical Cooperation Unit Nuclear Medicine, German Cancer Research Center, INF 280, 69120 Heidelberg, Germany
Department of Nuclear Medicine, University Hospital Heidelberg, INF 400, 69120 Heidelberg, Germany
Author to whom correspondence should be addressed.
Received: 23 December 2010 / Revised: 4 March 2011 / Accepted: 7 March 2011 / Published: 14 March 2011
(This article belongs to the Special Issue Phage Display of Combinatorial Libraries)
Download PDF [1214 KB, uploaded 18 June 2014]


Miniproteins are currently developed as alternative, non-immunoglobin proteins for the generation of novel binding motifs. Miniproteins are rigid scaffolds that are stabilised by alpha-helices, beta-sheets and disulfide-constrained secondary structural elements. They are tolerant to multiple amino acid substitutions, which allow for the integration of a randomised affinity function into the stably folded framework. These properties classify miniprotein scaffolds as promising tools for lead structure generation using phage display technologies. Owing to their high enzymatic resistance and structural stability, miniproteins are ideal templates to display binding epitopes for medical applications in vivo. This review summarises the characteristics and the engineering of miniproteins as a novel class of scaffolds to generate of alternative binding agents using phage display screening. Moreover, recent developments for therapeutic and especially diagnostic applications of miniproteins are reviewed.
Keywords: phage display; miniprotein; scaffold; in vivo diagnostics; protein engineering phage display; miniprotein; scaffold; in vivo diagnostics; protein engineering
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Zoller, F.; Haberkorn, U.; Mier, W. Miniproteins as Phage Display-Scaffolds for Clinical Applications. Molecules 2011, 16, 2467-2485.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top