Abstract: In the present study, we developed a liquid chromatography-diode array detector-electrospray ionization/mass spectrometric (LC-DAD-ESI/MS) method for analysis of saikosaponins in Bupleurum falcatum. The LC method employed a ZORBAX SB-Aq analytical column (150 × 4.6 mm i.d., 5 μm) at a flow rate of 0.8 mL/min coupled with a diode array detector at 204 nm. A step gradient of acetonitrile-water (v/v) containing 0.5% formic acid from 30 to 70% was applied, leading to a sample analysis time of 30 min. The ESI-MS was carried out in positive and negative modes from 500 to 1,500 m/z. Saikosaponins c, a, and d gave strong sodium adducts at m/z 949.6, 803.5 and 803.6, respectively, in positive mode. The data indicate that the present LC-DAD-ESI/MS assay is an effective method for the determination of saikosaponins c, a and d from the roots of Bupleurum falcatum.
Keywords: LC-DAD-ESI/MS; saikosaponins; pressurized liquid extraction; Bupleurum falcatum
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Zhao, L.-C.; Li, W.; He, Y.-F.; Li, X.-H.; Wang, Z.; Liu, W.-C.; Zheng, Y.-N.; Liang, J. Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu). Molecules 2011, 16, 1533-1543.
Zhao L-C, Li W, He Y-F, Li X-H, Wang Z, Liu W-C, Zheng Y-N, Liang J. Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu). Molecules. 2011; 16(2):1533-1543.
Zhao, Li-Chun; Li, Wei; He, Yu-Fang; Li, Xiao-Hua; Wang, Zi; Liu, Wen-Cong; Zheng, Yi-Nan; Liang, Jian. 2011. "Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu)." Molecules 16, no. 2: 1533-1543.