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Molecules 2011, 16(2), 1533-1543; doi:10.3390/molecules16021533

Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu)

2,†, 3, 4, 2, 2, 2 and 1,*
1 The Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College, Nanning 530011, China 2 College of Chinese Medicinal Material, Jilin Agricultural University, Changchun 130118, China 3 Jilin Academy of Chinese Medicine Sciences, Changchun 130012, China 4 Department of Crop Science, Chungnam National University, Daejeon 305-754, Korea These two authors contributed equally to this work and should be considered as co-first author.
* Author to whom correspondence should be addressed.
Received: 27 December 2010 / Revised: 17 January 2011 / Accepted: 20 January 2011 / Published: 11 February 2011
(This article belongs to the collection Bioactive Compounds)
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In the present study, we developed a liquid chromatography-diode array detector-electrospray ionization/mass spectrometric (LC-DAD-ESI/MS) method for analysis of saikosaponins in Bupleurum falcatum. The LC method employed a ZORBAX SB-Aq analytical column (150 × 4.6 mm i.d., 5 μm) at a flow rate of 0.8 mL/min coupled with a diode array detector at 204 nm. A step gradient of acetonitrile-water (v/v) containing 0.5% formic acid from 30 to 70% was applied, leading to a sample analysis time of 30 min. The ESI-MS was carried out in positive and negative modes from 500 to 1,500 m/z. Saikosaponins c, a, and d gave strong sodium adducts at m/z 949.6, 803.5 and 803.6, respectively, in positive mode. The data indicate that the present LC-DAD-ESI/MS assay is an effective method for the determination of saikosaponins c, a and d from the roots of Bupleurum falcatum.
Keywords: LC-DAD-ESI/MS; saikosaponins; pressurized liquid extraction; Bupleurum falcatum LC-DAD-ESI/MS; saikosaponins; pressurized liquid extraction; Bupleurum falcatum
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Zhao, L.-C.; Li, W.; He, Y.-F.; Li, X.-H.; Wang, Z.; Liu, W.-C.; Zheng, Y.-N.; Liang, J. Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu). Molecules 2011, 16, 1533-1543.

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