Next Article in Journal
Identification and Characterization of Three Novel Small Interference RNAs That Effectively Down-Regulate the Isolated Nucleocapsid Gene Expression of SARS Coronavirus
Next Article in Special Issue
Eucalyptus oleosa Essential Oils: Chemical Composition and Antimicrobial and Antioxidant Activities of the Oils from Different Plant Parts (Stems, Leaves, Flowers and Fruits)
Previous Article in Journal
Tannin Composition of Cabernet-Sauvignon and Merlot Grapes from the Bordeaux Area for Different Vintages (2006 to 2009) and Comparison to Tannin Profile of Five 2009 Vintage Mediterranean Grapes Varieties
Previous Article in Special Issue
Evaluation of the Bioactivity of Novel Spiroisoxazoline TypeCompounds against Normal and Cancer Cell Lines
Article Menu

Article Versions

Export Article

Open AccessArticle
Molecules 2011, 16(2), 1533-1543; doi:10.3390/molecules16021533

Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu)

1
The Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College, Nanning 530011, China
2
College of Chinese Medicinal Material, Jilin Agricultural University, Changchun 130118, China
3
Jilin Academy of Chinese Medicine Sciences, Changchun 130012, China
4
Department of Crop Science, Chungnam National University, Daejeon 305-754, Korea
These two authors contributed equally to this work and should be considered as co-first author.
*
Author to whom correspondence should be addressed.
Received: 27 December 2010 / Revised: 17 January 2011 / Accepted: 20 January 2011 / Published: 11 February 2011
(This article belongs to the Collection Bioactive Compounds)
Download PDF [225 KB, uploaded 18 June 2014]

Abstract

In the present study, we developed a liquid chromatography-diode array detector-electrospray ionization/mass spectrometric (LC-DAD-ESI/MS) method for analysis of saikosaponins in Bupleurum falcatum. The LC method employed a ZORBAX SB-Aq analytical column (150 × 4.6 mm i.d., 5 μm) at a flow rate of 0.8 mL/min coupled with a diode array detector at 204 nm. A step gradient of acetonitrile-water (v/v) containing 0.5% formic acid from 30 to 70% was applied, leading to a sample analysis time of 30 min. The ESI-MS was carried out in positive and negative modes from 500 to 1,500 m/z. Saikosaponins c, a, and d gave strong sodium adducts at m/z 949.6, 803.5 and 803.6, respectively, in positive mode. The data indicate that the present LC-DAD-ESI/MS assay is an effective method for the determination of saikosaponins c, a and d from the roots of Bupleurum falcatum.
Keywords: LC-DAD-ESI/MS; saikosaponins; pressurized liquid extraction; Bupleurum falcatum LC-DAD-ESI/MS; saikosaponins; pressurized liquid extraction; Bupleurum falcatum
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Zhao, L.-C.; Li, W.; He, Y.-F.; Li, X.-H.; Wang, Z.; Liu, W.-C.; Zheng, Y.-N.; Liang, J. Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu). Molecules 2011, 16, 1533-1543.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top