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Molecules 2011, 16(2), 1533-1543; doi:10.3390/molecules16021533
Article

Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu)

1,†
, 2,†, 3, 4, 2, 2, 2 and 1,*
Received: 27 December 2010; in revised form: 17 January 2011 / Accepted: 20 January 2011 / Published: 11 February 2011
(This article belongs to the collection Bioactive Compounds)
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Abstract: In the present study, we developed a liquid chromatography-diode array detector-electrospray ionization/mass spectrometric (LC-DAD-ESI/MS) method for analysis of saikosaponins in Bupleurum falcatum. The LC method employed a ZORBAX SB-Aq analytical column (150 × 4.6 mm i.d., 5 μm) at a flow rate of 0.8 mL/min coupled with a diode array detector at 204 nm. A step gradient of acetonitrile-water (v/v) containing 0.5% formic acid from 30 to 70% was applied, leading to a sample analysis time of 30 min. The ESI-MS was carried out in positive and negative modes from 500 to 1,500 m/z. Saikosaponins c, a, and d gave strong sodium adducts at m/z 949.6, 803.5 and 803.6, respectively, in positive mode. The data indicate that the present LC-DAD-ESI/MS assay is an effective method for the determination of saikosaponins c, a and d from the roots of Bupleurum falcatum.
Keywords: LC-DAD-ESI/MS; saikosaponins; pressurized liquid extraction; Bupleurum falcatum LC-DAD-ESI/MS; saikosaponins; pressurized liquid extraction; Bupleurum falcatum
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Zhao, L.-C.; Li, W.; He, Y.-F.; Li, X.-H.; Wang, Z.; Liu, W.-C.; Zheng, Y.-N.; Liang, J. Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu). Molecules 2011, 16, 1533-1543.

AMA Style

Zhao L-C, Li W, He Y-F, Li X-H, Wang Z, Liu W-C, Zheng Y-N, Liang J. Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu). Molecules. 2011; 16(2):1533-1543.

Chicago/Turabian Style

Zhao, Li-Chun; Li, Wei; He, Yu-Fang; Li, Xiao-Hua; Wang, Zi; Liu, Wen-Cong; Zheng, Yi-Nan; Liang, Jian. 2011. "Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu)." Molecules 16, no. 2: 1533-1543.


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