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Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu)
The Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College, Nanning 530011, China
College of Chinese Medicinal Material, Jilin Agricultural University, Changchun 130118, China
Jilin Academy of Chinese Medicine Sciences, Changchun 130012, China
Department of Crop Science, Chungnam National University, Daejeon 305-754, Korea
These two authors contributed equally to this work and should be considered as co-first author.
* Author to whom correspondence should be addressed.
Received: 27 December 2010; in revised form: 17 January 2011 / Accepted: 20 January 2011 / Published: 11 February 2011
Abstract: In the present study, we developed a liquid chromatography-diode array detector-electrospray ionization/mass spectrometric (LC-DAD-ESI/MS) method for analysis of saikosaponins in Bupleurum falcatum. The LC method employed a ZORBAX SB-Aq analytical column (150 × 4.6 mm i.d., 5 μm) at a flow rate of 0.8 mL/min coupled with a diode array detector at 204 nm. A step gradient of acetonitrile-water (v/v) containing 0.5% formic acid from 30 to 70% was applied, leading to a sample analysis time of 30 min. The ESI-MS was carried out in positive and negative modes from 500 to 1,500 m/z. Saikosaponins c, a, and d gave strong sodium adducts at m/z 949.6, 803.5 and 803.6, respectively, in positive mode. The data indicate that the present LC-DAD-ESI/MS assay is an effective method for the determination of saikosaponins c, a and d from the roots of Bupleurum falcatum.
Keywords: LC-DAD-ESI/MS; saikosaponins; pressurized liquid extraction; Bupleurum falcatum
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MDPI and ACS Style
Zhao, L.-C.; Li, W.; He, Y.-F.; Li, X.-H.; Wang, Z.; Liu, W.-C.; Zheng, Y.-N.; Liang, J. Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu). Molecules 2011, 16, 1533-1543.
Zhao L-C, Li W, He Y-F, Li X-H, Wang Z, Liu W-C, Zheng Y-N, Liang J. Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu). Molecules. 2011; 16(2):1533-1543.
Zhao, Li-Chun; Li, Wei; He, Yu-Fang; Li, Xiao-Hua; Wang, Zi; Liu, Wen-Cong; Zheng, Yi-Nan; Liang, Jian. 2011. "Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu)." Molecules 16, no. 2: 1533-1543.