Molecules 2008, 13(10), 2608-2627; doi:10.3390/molecules13102608

Surface-enhanced Raman Spectral Measurements of 5-Fluorouracil in Saliva

Received: 8 August 2008; in revised form: 16 October 2008 / Accepted: 20 October 2008 / Published: 22 October 2008
(This article belongs to the Special Issue 5-Fluorouracil)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The ability of surface-enhanced Raman spectroscopy (SERS) to measure 5-fluorouracil (5-FU) in saliva is presented. The approach is based on the capacity of Raman spectroscopy to provide a unique spectral signature for virtually every chemical, and the ability of SERS to provide μg/mL sensitivity. A simple sampling method, that employed 1-mm glass capillaries filled with silver-doped sol-gels, was developed to isolate 5-FU from potential interfering chemical components of saliva and simultaneously provide SERSactivity. The method involved treating a 1 mL saliva sample with 1 mL of acetic acid, drawing 10 μL of sample into a SERS-active capillary by syringe, and then measuring the SER spectrum. Quality SER spectra were obtained for samples containing as little as 2 μg of 5-FU in 1 mL saliva. The entire process, the acid pretreatment, extraction and spectral measurement, took less than 5 minutes. The SERS of 5-fluorouridine and 5-fluoro-2’-deoxyuridine, two major metabolites of 5-FU, were also measured and shown to have unique spectral peaks. These measurements suggest that disposable SERS-active capillaries could be used to measure 5-FU and metabolite concentrations in chemotherapy patient saliva, thereby providing metabolic data that would allow regulating dosage. Tentative vibrational mode assignments for 5-FU and its metabolites are also given.
Keywords: 5-FU; Chemotherapy; Drug analysis; Saliva analysis; Raman; SERS
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MDPI and ACS Style

Farquharson, S.; Gift, A.; Shende, C.; Inscore, F.; Ordway, B.; Farquharson, C.; Murren, J. Surface-enhanced Raman Spectral Measurements of 5-Fluorouracil in Saliva. Molecules 2008, 13, 2608-2627.

AMA Style

Farquharson S, Gift A, Shende C, Inscore F, Ordway B, Farquharson C, Murren J. Surface-enhanced Raman Spectral Measurements of 5-Fluorouracil in Saliva. Molecules. 2008; 13(10):2608-2627.

Chicago/Turabian Style

Farquharson, Stuart; Gift, Alan; Shende, Chetan; Inscore, Frank; Ordway, Beth; Farquharson, Carl; Murren, John. 2008. "Surface-enhanced Raman Spectral Measurements of 5-Fluorouracil in Saliva." Molecules 13, no. 10: 2608-2627.

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