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Molecules 2008, 13(11), 2729-2739; doi:10.3390/molecules13112729
Article

Influence of Spray-dried Hydroxyapatite-5-Fluorouracil Granules on Cell Lines Derived from Tissues of Mesenchymal Origin

1,* , 1,2
, 1,3
, 2
 and 2
Received: 8 June 2008; in revised form: 9 October 2008 / Accepted: 24 October 2008 / Published: 1 November 2008
(This article belongs to the Special Issue 5-Fluorouracil)
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Abstract: In our previous work we described the preparation and characterization of spray dried hydroxyapatite micro granules loaded with 5-fluorouracil (5-FU). These loaded particles are used as a model drug delivery system (DDS). In this study we examined the in vitro response of two cell lines derived from different tissues to 5-FU loaded granules (LG). Both cell lines, either L929 cells of a mouse fibroblast lineage or cells originating from a rat osteosarcoma (ROS 17/2.8) showed a dose dependent decrease in cell proliferation in response to 5-FU-, either dissolved in the culture medium or loaded onto particles. The response of the two cell lines to loaded and nonloaded particles was different. The effect of LG and of a corresponding concentration of free 5-FU was practically the same for the ROS 17/2.8 cells indicating that ROS 17/2.8 cells were not affected by the carrier material. In contrast, L929 cells showed a slight decrease in cell proliferation also in the presence of granules not loaded with 5-FU. This is thought to be attributed to the inhibition of mitogenesis by phosphocitrates, already demonstrated in fibroblasts. In summary, we found that the loaded 5-FU kept its effectivity after the spray drying process and that the response towards the granules varied with cell type. This is the first step towards a tissue specific DDS.
Keywords: Hydroxyapatite; Spray dried particles; 5-Fluorouracil; in Vitro cytotoxicity; Drug delivery system Hydroxyapatite; Spray dried particles; 5-Fluorouracil; in Vitro cytotoxicity; Drug delivery system
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Scharnweber, T.; Santos, C.; Franke, R.-P.; Almeida, M.M.; Costa, M.E.V. Influence of Spray-dried Hydroxyapatite-5-Fluorouracil Granules on Cell Lines Derived from Tissues of Mesenchymal Origin. Molecules 2008, 13, 2729-2739.

AMA Style

Scharnweber T, Santos C, Franke R-P, Almeida MM, Costa MEV. Influence of Spray-dried Hydroxyapatite-5-Fluorouracil Granules on Cell Lines Derived from Tissues of Mesenchymal Origin. Molecules. 2008; 13(11):2729-2739.

Chicago/Turabian Style

Scharnweber, Tim; Santos, Catarina; Franke, Ralf-Peter; Almeida, Maria M.; Costa, Maria Elisabete V. 2008. "Influence of Spray-dried Hydroxyapatite-5-Fluorouracil Granules on Cell Lines Derived from Tissues of Mesenchymal Origin." Molecules 13, no. 11: 2729-2739.



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