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Influence of Spray-dried Hydroxyapatite-5-Fluorouracil Granules on Cell Lines Derived from Tissues of Mesenchymal Origin
Institute for Biological Interfaces; Forschungszentrum Karlsruhe GmbH, 76344 Eggenstein- Leopoldshafen Karlsruhe, Germany
Department of Ceramic and Glass Engineering, CICECO, University of Aveiro, 3810-193 Aveiro, Portugal
Department of Biomaterials, University of Ulm, 89081 Ulm, Germany
* Author to whom correspondence should be addressed.
Received: 8 June 2008; in revised form: 9 October 2008 / Accepted: 24 October 2008 / Published: 1 November 2008
Abstract: In our previous work we described the preparation and characterization of spray dried hydroxyapatite micro granules loaded with 5-fluorouracil (5-FU). These loaded particles are used as a model drug delivery system (DDS). In this study we examined the in vitro response of two cell lines derived from different tissues to 5-FU loaded granules (LG). Both cell lines, either L929 cells of a mouse fibroblast lineage or cells originating from a rat osteosarcoma (ROS 17/2.8) showed a dose dependent decrease in cell proliferation in response to 5-FU-, either dissolved in the culture medium or loaded onto particles. The response of the two cell lines to loaded and nonloaded particles was different. The effect of LG and of a corresponding concentration of free 5-FU was practically the same for the ROS 17/2.8 cells indicating that ROS 17/2.8 cells were not affected by the carrier material. In contrast, L929 cells showed a slight decrease in cell proliferation also in the presence of granules not loaded with 5-FU. This is thought to be attributed to the inhibition of mitogenesis by phosphocitrates, already demonstrated in fibroblasts. In summary, we found that the loaded 5-FU kept its effectivity after the spray drying process and that the response towards the granules varied with cell type. This is the first step towards a tissue specific DDS.
Keywords: Hydroxyapatite; Spray dried particles; 5-Fluorouracil; in Vitro cytotoxicity; Drug delivery system
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Scharnweber, T.; Santos, C.; Franke, R.-P.; Almeida, M.M.; Costa, M.E.V. Influence of Spray-dried Hydroxyapatite-5-Fluorouracil Granules on Cell Lines Derived from Tissues of Mesenchymal Origin. Molecules 2008, 13, 2729-2739.
Scharnweber T, Santos C, Franke R-P, Almeida MM, Costa MEV. Influence of Spray-dried Hydroxyapatite-5-Fluorouracil Granules on Cell Lines Derived from Tissues of Mesenchymal Origin. Molecules. 2008; 13(11):2729-2739.
Scharnweber, Tim; Santos, Catarina; Franke, Ralf-Peter; Almeida, Maria M.; Costa, Maria Elisabete V. 2008. "Influence of Spray-dried Hydroxyapatite-5-Fluorouracil Granules on Cell Lines Derived from Tissues of Mesenchymal Origin." Molecules 13, no. 11: 2729-2739.