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KeywordsE. coli SHuffle® T7; Glycosyltransferase GalNAcT2; In Situ monitoring; soft sensor; fluorescence spectroscopy; multivariate curve resolution; E. coli; fed-batch; fermentation; carbon mass balance constraint; soft constraints; alternating least squares; hybrid modelling; State estimation; hybrid modeling; Unscented Kalman Filter; recombinant protein production; computational fluid dynamics; cell cycle model; Lagrange trajectory; scale-up; stirred tank reactor; population dynamics; energy level; mesenchymal stem cells; scaffold-free; aggregate cultivation; stirred tank reactor; dynamic cultivation; hypoxia; stemness; computational fluid dynamics; perfusion bioreactor system; 3D cell culture; dynamic cultivation; fluid shear stress; computational fluid dynamics; antibody aggregation; ANS; bioprocess monitoring; Bis-ANS; fluorescence spectroscopy; CHO; retrospective power analysis; process characterization study; process validation stage 1; criticality assessment; control strategy; design of experiments; process validation; process characterization study; holistic process model; predict out of specification events; Monte Carlo simulation; biopharmaceutical manufacturing; E. coli; mixed feed system; glycerol; recombinant proteins; bioprocess engineering
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