Special Issue "Advantages of Three Dimensional (3D) Cell Cultures"
A special issue of Microarrays (ISSN 2076-3905).
Deadline for manuscript submissions: closed (30 June 2015)
In the last years progress has been made that the common technique where adherent cells grow in monolayers in two-dimensional (2D) cell culture has its limitations. When cultured in the long-term in 2D, the supply of nutrients is insufficient, the cell-cell and cell-material interactions are unphysiological and most of the cells will not stay viable. The upcoming three-dimensional (3D) cell culture models promise to overcome these disadvantages and should resemble more likely the in vivo situation of tissues or organs. Currently, many different 3D cell culture models using different cell types, conditions and materials are under investigation. All of them is common that they must improve their advantages for basic and applied research. Microarrays can be important tools to analyze the characteristic gene expression profiles of the 3D cell culture models, doing comparative studies, and to define the 3D culture effects. The special issue invites contributions to publish their results about gene expression profiling in different 3D cell culture models.
It will be of interest to the readers of this special issue to show, how 3D cell culture has influence on cell proliferation, cell differentiation, cell viability, general cell functionality, response to stimuli and in metabolism in comparison to highlight its advantages for basic and applied research.
Dr. Mohammad Reza Lornejad-Schafer
Dr. Christine Schäfer
Manuscript Submission Information
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microarrays is an international peer-reviewed open access quarterly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 350 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.
- gene expression
- three-dimensional (3D) cell culture
- two-dimensional (2D) cell culture
- in vitro model
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Type of Paper: Article
Title: 3D-Cell Culture System in Tumor Biology and Drug Screening in Due Consideration of the Microenvironment
Author: Gerhard Unteregger *
Affiliation: University of the Saar, Clinic of Urology, Homburg7Saar, Germany; E-Mail: Gerhard.Unteregger@uniklinikum-saarland.de
Abstract: "Good bye flat cell biology" claims for three-dimensional experimental designs in cell culture to mimic heterologeous cell-cell and cell-matrix interaction. Since the discovery in the late 70th that fibroblasts and extracellular matrix are indispensable components to build up reliable in vitro skin equivalents the necessity to include the environment in the experimental design is well acknowledged even in tumor biology. Tumour-associated and activated Fibroblasts, extracellular matrix and secreted proteins define in a well balanced communication and interaction tissue and organ development. The meaning of the surrounding environment in prostate cancer initiation and -progression was first described more than 20 years ago. During the last decades the general meaning of especially tumor-associated fibroblasts in tumor biology in several entities like breast, lung and bladder cancer was confirmed. Any genetic or epigenetic changes in these partners lead to functional changes which finally dictate the malignant process - even during patients relapse. Thus, standard monolayer cell cultures represent reduced models in epithelial cancer because they lack 3D architecture, cell-ECM interaction and fail to simulate paracrine mechanisms. One pivotal goal of in vitro studies in tumor biology and medicine is to reveal new therapeutic targets even under consideration of the surrounding environment to evaluate the efficacy of drug application and finally to disclose the molecular mechanism leading to drug resistance. Consequently, several strategies were developed to overcome the limitations of monolayer cell culture and to produce reliable, in vivo like and screening-compatible homo- and heterologeous 3D-tumor cultures to close the gap between in vitro systems and animal models. We focused our strategy on 3D- models on prostate cancer, bladder cancer and renal cell carcinoma cell lines. To build up the biological situation we combined tumor cells with tumor associated fibroblast to produce 3D-spheroids on standard 96-well plates. To avoid harvesting of spheroids for confocal microscopy we used standard assays to quantify cell growth and death. Using this approach we investigated the impact of nanomodified Zoledronic acid on 3D-spheroids from prostate carcinoma cells. Our results confirmed the assumption that there are remarkable differences in the efficacy of the drug in 3D-spheres compared to the flat cell system. Additionally, using patients derived primary prostate cancer specimens we established and 3D-invasion system to collect invasive growing tumor cells. Our results offer remarkable constant genetic imbalance only in the invasive growing 3D-spheres by CGH-analysis. In summary, 3D-cell culture technology offers an innovative powerful tool in cancer research.